{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:21:32Z","timestamp":1791177692686,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,12]],"date-time":"2013-11-12T00:00:00Z","timestamp":1384214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>The cellular adaptive immune system mounts a response to many solid tumours mediated by tumour\u2010infiltrating T lymphocytes (<jats:styled-content style=\"fixed-case\">TILs<\/jats:styled-content>). Basic measurements of these <jats:styled-content style=\"fixed-case\">TILs<\/jats:styled-content>, including total count, show promise as prognostic markers for a variety of cancers, including ovarian and colorectal. In addition, recent therapeutic advances are thought to exploit this immune response to effectively fight melanoma, with promising studies showing efficacy in additional cancers. However, many of the basic properties of <jats:styled-content style=\"fixed-case\">TILs<\/jats:styled-content> are poorly understood, including specificity, clonality, and spatial heterogeneity of the T\u2010cell response. We utilize deep sequencing of rearranged T\u2010cell receptor beta (<jats:italic><jats:styled-content style=\"fixed-case\">TCRB<\/jats:styled-content><\/jats:italic>) genes to characterize the basic properties of <jats:styled-content style=\"fixed-case\">TILs<\/jats:styled-content> in ovarian carcinoma. Due to somatic rearrangement during T\u2010cell development, the <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content> beta chain sequence serves as a molecular tag for each T\u2010cell clone. Using these sequence tags, we assess similarities and differences between infiltrating T cells in discretely sampled sections of large tumours and compare to T cells from peripheral blood. Within the limits of sensitivity of our assay, the <jats:styled-content style=\"fixed-case\">TIL<\/jats:styled-content> repertoires show strong similarity throughout each tumour and are distinct from the circulating T\u2010cell repertoire. We conclude that the cellular adaptive immune response within ovarian carcinomas is spatially homogeneous and distinct from the T\u2010cell compartment of peripheral blood. Copyright \u00a9 2013 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:bold><\/jats:p>","DOI":"10.1002\/path.4260","type":"journal-article","created":{"date-parts":[[2013,9,11]],"date-time":"2013-09-11T15:26:46Z","timestamp":1378913206000},"page":"433-440","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":139,"title":["High\u2010throughput sequencing of T\u2010cell receptors reveals a homogeneous repertoire of tumour\u2010infiltrating lymphocytes in ovarian cancer"],"prefix":"10.1002","volume":"231","author":[{"given":"Ryan O","family":"Emerson","sequence":"first","affiliation":[{"name":"Adaptive Biotechnologies Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna M","family":"Sherwood","sequence":"additional","affiliation":[{"name":"Adaptive Biotechnologies Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J","family":"Rieder","sequence":"additional","affiliation":[{"name":"Adaptive 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