{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T05:31:17Z","timestamp":1770701477867,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T00:00:00Z","timestamp":1390780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Loss of mitotic checkpoint of cells contributes to chromosomal instability and leads to carcinogenesis. Mitotic arrest deficient 1 (<jats:styled-content style=\"fixed-case\">MAD1<\/jats:styled-content>) is a key component in mitotic checkpoint signalling. In this study, we identified a novel <jats:styled-content style=\"fixed-case\">MAD1<\/jats:styled-content> interacting partner, proline\u2010rich acidic protein 1 (<jats:styled-content style=\"fixed-case\">PRAP1<\/jats:styled-content>), using yeast\u2010two hybrid screening, and investigated its role in mitotic checkpoint signalling in hepatocellular carcinoma (<jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>). We demonstrated the physical interaction of <jats:styled-content style=\"fixed-case\">PRAP1<\/jats:styled-content> with <jats:styled-content style=\"fixed-case\">MAD1<\/jats:styled-content> and of <jats:styled-content style=\"fixed-case\">PRAP1<\/jats:styled-content> with <jats:styled-content style=\"fixed-case\">MAD1<\/jats:styled-content> isoform <jats:styled-content style=\"fixed-case\">MAD1<\/jats:styled-content><jats:italic>\u03b2<\/jats:italic>, using a co\u2010immunoprecipitation assay. Moreover, stable expression of <jats:styled-content style=\"fixed-case\">PRAP1<\/jats:styled-content> in mitotic checkpoint\u2010competent <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> cells, <jats:styled-content style=\"fixed-case\">BEL<\/jats:styled-content>\u20107402 and <jats:styled-content style=\"fixed-case\">SMMC<\/jats:styled-content>\u20107721, induced impairment of the mitotic checkpoint (<jats:italic>p<\/jats:italic> &lt; 0.01), formation of chromosome bridges (<jats:italic>p<\/jats:italic> &lt; 0.01) and aberrant chromosome numbers (<jats:italic>p<\/jats:italic> &lt; 0.001). Interestingly, ectopic expression <jats:styled-content style=\"fixed-case\">PRAP1<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> cells led to significant under\u2010expression of <jats:styled-content style=\"fixed-case\">MAD1<\/jats:styled-content>. In human <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> tumours, 40.4% (23\/57) of <jats:styled-content style=\"fixed-case\">HCCs<\/jats:styled-content> showed under\u2010expression of <jats:styled-content style=\"fixed-case\">PRAP1<\/jats:styled-content> protein as compared with their corresponding non\u2010tumorous livers; up\u2010regulation of <jats:styled-content style=\"fixed-case\">MAD1<\/jats:styled-content> protein was significantly associated with down\u2010regulation of <jats:styled-content style=\"fixed-case\">PRAP1<\/jats:styled-content> (<jats:italic>p<\/jats:italic> = 0.030). Our data revealed that <jats:styled-content style=\"fixed-case\">PRAP1<\/jats:styled-content> is a protein interacting partner of <jats:styled-content style=\"fixed-case\">MAD1<\/jats:styled-content> and that <jats:styled-content style=\"fixed-case\">PRAP1<\/jats:styled-content> is able to down\u2010regulate <jats:styled-content style=\"fixed-case\">MAD1<\/jats:styled-content> and suppress mitotic checkpoint signalling in <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>. Published by John Wiley &amp; Sons, Ltd. <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.pathsoc.org.uk\">www.pathsoc.org.uk<\/jats:ext-link><\/jats:p>","DOI":"10.1002\/path.4319","type":"journal-article","created":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T10:34:43Z","timestamp":1388054083000},"page":"51-60","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Proline\u2010rich acidic protein 1 (<scp>PRAP1<\/scp>) is a novel interacting partner of <scp>MAD1<\/scp> and has a suppressive role in mitotic checkpoint signalling in hepatocellular carcinoma"],"prefix":"10.1002","volume":"233","author":[{"given":"Karen Man\u2010Fong","family":"Sze","sequence":"first","affiliation":[{"name":"State Key Laboratory for Liver Research University of Hong Kong"},{"name":"Department of Pathology University of Hong 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