{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:09:46Z","timestamp":1791295786962,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,1,23]],"date-time":"2017-01-23T00:00:00Z","timestamp":1485129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI14\/02043"],"award-info":[{"award-number":["PI14\/02043"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2017,3]]},"abstract":"Abstract<\/jats:title>Endometrial cancer is the most common gynaecological cancer in western countries, being the most common subtype of endometrioid tumours. Most patients are diagnosed at an early stage and present an excellent prognosis. However, a number of those continue to suffer recurrence, without means of identification by risk classification systems. Thus, finding a reliable marker to predict recurrence becomes an important unmet clinical issue. ALCAM<\/jats:styled-content> is a cell\u2013cell adhesion molecule and member of the immunoglobulin superfamily that has been associated with the genesis of many cancers. Here, we first determined the value of ALCAM<\/jats:styled-content> as a marker of recurrence in endometrioid endometrial cancer by conducting a retrospective multicentre study of 174 primary tumours. In early\u2010stage patients (N<\/jats:italic> = 134), recurrence\u2010free survival was poorer in patients with ALCAM<\/jats:styled-content>\u2010positive compared to ALCAM<\/jats:styled-content>\u2010negative tumours (HR<\/jats:styled-content> 4.237; 95% CI<\/jats:styled-content> 1.01\u201317.76). This difference was more significant in patients with early\u2010stage moderately\u2013poorly differentiated tumours (HR<\/jats:styled-content> 9.259; 95% CI<\/jats:styled-content> 2.12\u201353.47). In multivariate analysis, ALCAM<\/jats:styled-content> positivity was an independent prognostic factor in early\u2010stage disease (HR<\/jats:styled-content> 6.027; 95% CI<\/jats:styled-content> 1.41\u201325.74). Then we demonstrated in vitro<\/jats:italic> a role for ALCAM<\/jats:styled-content> in cell migration and invasion by using a loss\u2010of\u2010function model in two endometrial cancer cell lines. ALCAM<\/jats:styled-content> depletion resulted in a reduced primary tumour size and reduced metastatic local spread in an orthotopic murine model. Gene expression analysis of ALCAM\u2010depleted cell lines pointed to motility, invasiveness, cellular assembly, and organization as the most deregulated functions. Finally, we assessed some of the downstream effector genes that are involved in ALCAM<\/jats:styled-content>\u2010mediated cell migration; specifically FLNB<\/jats:styled-content><\/jats:italic>, TXNRD1<\/jats:styled-content><\/jats:italic>, and LAMC2<\/jats:styled-content><\/jats:italic> were validated at the mRNA<\/jats:styled-content> and protein level. In conclusion, our results highlight the potential of ALCAM<\/jats:styled-content> as a recurrent biomarker in early\u2010stage endometrioid endometrial cancer and point to ALCAM<\/jats:styled-content> as an important molecule in endometrial cancer dissemination by regulating cell migration, invasion, and metastasis. Copyright \u00a9 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.4851","type":"journal-article","created":{"date-parts":[[2016,11,22]],"date-time":"2016-11-22T01:23:01Z","timestamp":1479777781000},"page":"475-487","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Activated leukocyte cell adhesion molecule (ALCAM<\/scp>) is a marker of recurrence and promotes cell migration, invasion, and metastasis in early\u2010stage endometrioid endometrial cancer"],"prefix":"10.1002","volume":"241","author":[{"given":"Laura","family":"Devis","sequence":"first","affiliation":[{"name":"Biomedical Research Group in Gynecology, Vall Hebron Research Institute (VHIR) Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristian P","family":"Moiola","sequence":"additional","affiliation":[{"name":"Biomedical Research Group in Gynecology, Vall Hebron Research Institute (VHIR) Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuria","family":"Masia","sequence":"additional","affiliation":[{"name":"Cell Cycle and Ovarian Cancer Group, Biomedical Research Group in Gynecology, Vall Hebron Research Institute (VHIR) Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Martinez\u2010Garcia","sequence":"additional","affiliation":[{"name":"Biomedical Research Group in Gynecology, Vall Hebron Research Institute (VHIR) Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Santacana","sequence":"additional","affiliation":[{"name":"Pathological Oncology Group and Pathology Department Hospital Arnau de Vilanova Lleida Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomita Vasilica","family":"Stirbat","sequence":"additional","affiliation":[{"name":"Institut Curie, CNRS, UMR168 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Brochard\u2010Wyart","sequence":"additional","affiliation":[{"name":"Institut Curie, CNRS, UMR168 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngel","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Pathology Department Vall Hebron University Hospital Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesc","family":"Alameda","sequence":"additional","affiliation":[{"name":"Pathology Department Hospital del Mar Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Cabrera","sequence":"additional","affiliation":[{"name":"Gynecological Oncology Department Vall Hebron University Hospital Barcelona 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Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Natural Sciences, Colby\u2010Sawyer College New London NH 03257 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Dufour","sequence":"additional","affiliation":[{"name":"Institut Curie, CNRS, UMR144 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Matias\u2010Guiu","sequence":"additional","affiliation":[{"name":"Pathological Oncology Group and Pathology Department Hospital Arnau de Vilanova Lleida Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Santamaria","sequence":"additional","affiliation":[{"name":"Cell Cycle and Ovarian Cancer Group, Biomedical Research Group in Gynecology, Vall Hebron Research Institute (VHIR) Universitat Aut\u00f2noma de Barcelona Barcelona 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