{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:58:12Z","timestamp":1790506692374,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,6,28]],"date-time":"2018-06-28T00:00:00Z","timestamp":1530144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U10 CA180790"],"award-info":[{"award-number":["U10 CA180790"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1 TR000135"],"award-info":[{"award-number":["UL1 TR000135"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In patients with metastatic melanoma, high blood levels of galectin\u20109 are correlated with worse overall survival and a bias towards a Th2 inflammatory state supportive of tumor growth. Although galectin\u20109 signaling through TIM3 on T cells has been described, less is known about the interaction of galectin\u20109 with macrophages. We aimed to determine whether galectin\u20109 is a binding partner of CD206 on macrophages and whether the result of this interaction is tumor\u2010supportive. It was determined that incubation of CD68\n                    <jats:sup>+<\/jats:sup>\n                    macrophages with galectin\u20109 or anti\u2010CD206 blocked target binding and that both CD206 and galectin\u20109 were detected by immunoprecipitation of cell lysates. CD206 and galectin\u20109 had a binding affinity of 2.8 \u00d7 10\n                    <jats:sup>\u22127<\/jats:sup>\n                    \u00a0\n                    <jats:sc>m<\/jats:sc>\n                    . Galectin\u20109 causes CD206\n                    <jats:sup>+<\/jats:sup>\n                    macrophages to make significantly more FGF2 and monocyte chemoattractant protein (MCP\u20101), but less macrophage\u2010derived chemokine (MDC). Galectin\u20109 had no effect on classical monocyte subsets, but caused expansion of the non\u2010classical populations. Lastly, there was a positive correlation between increasing numbers of CD206 macrophages and galectin\u20109 expression in tumors, and high levels of CD206 macrophages correlated negatively with melanoma survival. These results indicate that galectin\u20109 binds to CD206 on M2 macrophages, which appear to drive angiogenesis and the production of chemokines that support tumor growth and poor patient prognoses. Targeting this interaction systemically through circulating monocytes may therefore be a novel way to improve local anti\u2010tumor effects by macrophages. Copyright \u00a9 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/path.5093","type":"journal-article","created":{"date-parts":[[2018,5,7]],"date-time":"2018-05-07T04:34:42Z","timestamp":1525667682000},"page":"468-477","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["CD206\u2010positive myeloid cells bind galectin\u20109 and promote a tumor\u2010supportive microenvironment"],"prefix":"10.1002","volume":"245","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3621-7335","authenticated-orcid":false,"given":"Elizabeth Ann L","family":"Enninga","sequence":"first","affiliation":[{"name":"Department of Oncology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyriakos","family":"Chatzopoulos","sequence":"additional","affiliation":[{"name":"Department of Anatomic Pathology Mayo Clinic  Rochester Minnesota 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