{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:57:40Z","timestamp":1787061460736,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T00:00:00Z","timestamp":1545091200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001381","name":"National Research Foundation Singapore","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001381","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The evaluation of intra\u2010tumour heterogeneity (ITH) from a transcriptomic point of view is limited. Single\u2010cell cancer studies reveal significant genomic and transcriptomic ITH within a tumour and it is no longer adequate to employ single\u2010subtype assignment as this does not acknowledge the ITH that exists. Molecular assessment of subtype heterogeneity (MASH) was developed to comprehensively report on the composition of all transcriptomic subtypes within a tumour lesion. Using MASH on 3431 ovarian cancer samples, correlation and association analyses with survival, metastasis and clinical outcomes were performed to assess the impact of subtype composition as a surrogate for ITH. The association was validated on two independent cohorts. We identified that 30% of ovarian tumours consist of two or more subtypes. When biological features of the subtype constituents were examined, we identified significant impact on clinical outcomes with the presence of poor prognostic subtypes (Mes or Stem\u2010A). Poorer outcomes correlated with having higher degrees of poor prognostic subtype populations within the tumour. Subtype prediction in several independent datasets reflected a similar prognostic trend. In addition, paired analysis of primary and recurrent\/metastatic tumours demonstrated Mes and\/or Stem\u2010A subtypes predominated in recurrent and metastatic tumours regardless of the original primary subtype. Given the biological and prognostic value in delineating individual subtypes within a tumour, a clinically applicable MASH assay using NanoString\n                    <jats:sup>\u00ae<\/jats:sup>\n                    technology was developed as a classification tool to comprehensively describe constituents of molecular subtypes. Copyright \u00a9 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/path.5191","type":"journal-article","created":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T08:42:01Z","timestamp":1540888921000},"page":"305-319","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Decoding transcriptomic intra\u2010tumour heterogeneity to guide personalised medicine in ovarian cancer"],"prefix":"10.1002","volume":"247","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6624-1593","authenticated-orcid":false,"given":"Tuan Zea","family":"Tan","sequence":"first","affiliation":[{"name":"Cancer Science Institute of Singapore, National University of Singapore, Center for Translational Medicine  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Heong","sequence":"additional","affiliation":[{"name":"Cancer Science Institute of Singapore, National University of Singapore, Center for Translational Medicine  Singapore"},{"name":"Department of Haematology\u2010Oncology National University Cancer Institute Singapore, Level 8 NUH Medical Center  Singapore"},{"name":"Walter and Eliza Hall Institute of Medical Research  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieru","family":"Ye","sequence":"additional","affiliation":[{"name":"Cancer Science Institute of Singapore, National University of Singapore, Center for Translational Medicine  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Pathology National University Health System  Singapore"},{"name":"Department of Pathology, Yong Loo Lin School of Medicine National University of Singapore  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Low","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology National University Health System  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahesh","family":"Choolani","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology National University Health System  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare","family":"Scott","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David Shao Peng","family":"Tan","sequence":"additional","affiliation":[{"name":"Cancer Science Institute of Singapore, National University of Singapore, Center for Translational Medicine  Singapore"},{"name":"Department of Haematology\u2010Oncology National University Cancer Institute Singapore, Level 8 NUH Medical Center  Singapore"},{"name":"Department of Medicine, Yong Loo Lin School of Medicine National 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