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However, various locations and proliferative activities of gastric stem cells have been reported. We focused here on the stem cell marker Bmi1, a polycomb group protein, aiming to elucidate the characteristics of Bmi1\u2010expressing cells in the stomach and to examine their stem cell potential. We investigated the Bmi1\u2010expressing cell lineage in <jats:italic>Bmi1\u2010CreERT; Rosa26\u2010YFP, LacZ<\/jats:italic> or <jats:italic>Rosa26\u2010Confetti<\/jats:italic> mice. We examined the <jats:italic>in vivo<\/jats:italic> and <jats:italic>ex vivo<\/jats:italic> effects of Bmi1\u2010expressing cell ablation by using <jats:italic>Bmi1\u2010CreERT; Rosa26\u2010iDTR<\/jats:italic> mice. The Bmi1 lineage was also traced during regeneration after high\u2010dose tamoxifen\u2010, irradiation\u2010 and acetic acid\u2010induced mucosal injuries. In the lineage\u2010tracing experiments using low\u2010dose tamoxifen, Bmi1\u2010expressing cells in the isthmus of the gastric antrum and corpus provided progeny bidirectionally, towards both the luminal and basal sides over 6\u2009months. In gastric organoids, Bmi1\u2010expressing cells also provided progeny. Ablation of Bmi1\u2010expressing cells resulted in impaired gastric epithelium in both mouse stomach and organoids. After high\u2010dose tamoxifen\u2010induced gastric mucosal injury, Bmi1\u2010expressing cell lineages expanded and fully occupied all gastric glands of the antrum and the corpus within 7\u2009days after tamoxifen injection. After irradiation\u2010 and acetic acid\u2010induced gastric mucosal injuries, Bmi1\u2010expressing cells also contributed to regeneration. In conclusion, Bmi1 is a gastric stem cell marker expressed in the isthmus of the antrum and corpus. Bmi1\u2010expressing cells have stem cell potentials, both under physiological conditions and during regeneration after gastric mucosal injuries. Copyright \u00a9 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.5244","type":"journal-article","created":{"date-parts":[[2019,1,28]],"date-time":"2019-01-28T16:43:21Z","timestamp":1548693801000},"page":"179-190","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Bmi1 marks gastric stem cells located in the isthmus in mice"],"prefix":"10.1002","volume":"248","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1097-6632","authenticated-orcid":false,"given":"Takuto","family":"Yoshioka","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kyoto University Graduate School of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihisa","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kyoto University Graduate School of Medicine  Kyoto 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