{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:33:10Z","timestamp":1788827590068,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T00:00:00Z","timestamp":1574985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Endosalpingiosis, a microscopic lesion composed of ectopic Fallopian tube epithelium, frequently involves the peritoneum and lymph nodes in patients with ovarian serous borderline tumour or low\u2010grade serous carcinoma, but its pathogenic significance remains unclear. Using laser\u2010capture microdissection and droplet digital PCR, we investigated whether endosalpingiosis harbours the driver mutations in <jats:italic>BRAF<\/jats:italic> and <jats:italic>KRAS<\/jats:italic> that characterise ovarian low\u2010grade serous neoplasms. Somatic mutations were detected in 14 (33%) of 43 endosalpingiotic lesions analysed. Of 21 women with endosalpingiosis associated with a synchronous or metachronous ovarian low\u2010grade serous tumour, mutations were identified in endosalpingiotic lesions from 11 (52%) women, with most cases (10\/11, 91%) demonstrating identical mutations in both tumour and endosalpingiosis. In contrast, of 13 cases of endosalpingiosis not associated with an ovarian tumour, only one harboured a <jats:italic>KRAS<\/jats:italic> mutation. The proliferative activity as assessed by Ki\u201067 immunohistochemistry was lower in endosalpingiosis than in low\u2010grade serous tumours, and endosalpingiosis with either a <jats:italic>BRAF<\/jats:italic> or <jats:italic>KRAS<\/jats:italic> mutation had a significantly lower Ki\u201067 index than those without. Ectopic expression of KRAS<jats:sup>G12V<\/jats:sup> in Fallopian tube epithelial cells led to ERK phosphorylation, p21 induction, growth arrest and cellular senescence. In conclusion, we demonstrate that endosalpingiosis represents an interesting example of cancer driver mutations in deceptively normal\u2010appearing cells, which may be prone to neoplastic transformation upon bypass of endogenous oncosuppressive mechanisms. \u00a9 2019 Pathological Society of Great Britain and Ireland. 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