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Human cytomegalovirus (HCMV), which infects 50\u201380% of humans, is usually acquired during early life and persists in a latent state for the life of the individual. In view of its previously described pro\u2010angiogenic properties, we hypothesized that cytomegalovirus might be a novel risk factor for progression to an advanced form, neovascular AMD, which is characterized by choroidal neovascularization (CNV). The purpose of this study was to investigate if latent ocular murine cytomegalovirus (MCMV) infection exacerbated the development of CNV in vascular endothelial growth factor (VEGF)\u2010overexpressing VEGF\u2010A<jats:sup>hyper<\/jats:sup> mice. Here we show that neonatal infection with MCMV resulted in dissemination of virus to various organs throughout the body including the eye, where it localized principally to the choroid in both VEGF\u2010overexpressingVEGF\u2010A<jats:sup>hyper<\/jats:sup> and wild\u2010type(WT) 129 mice. By 6 months post\u2010infection, no replicating virus was detected in eyes and extraocular tissues, although virus DNA was still present in all eyes and extraocular tissues of both VEGF\u2010A<jats:sup>hyper<\/jats:sup> and WT mice. Expression of MCMV immediate early (IE) 1 mRNA was detected only in latently infected eyes of VEGF\u2010A<jats:sup>hyper<\/jats:sup> mice, but not in eyes of WT mice. Significantly increased CNV was observed in eyes of MCMV\u2010infected VEGF\u2010A<jats:sup>hyper<\/jats:sup> mice compared to eyes of uninfected VEGF\u2010A<jats:sup>hyper<\/jats:sup> mice, while no CNV lesions were observed in eyes of either infected or uninfected WT mice. Protein levels of several inflammatory\/angiogenic factors, particularly VEGF and IL\u20106, were significantly higher in eyes of MCMV\u2010infected VEGF\u2010A<jats:sup>hyper<\/jats:sup> mice, compared to uninfected controls. Initial studies of ocular tissue from human cadavers revealed that HCMV DNA was present in four choroid\/retinal pigment epithelium samples from 24 cadavers. Taken together, our data suggest that ocular HCMV latency could be a significant risk factor for the development of AMD. \u00a9 2020 Pathological Society of Great Britain and Ireland. 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