{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T08:57:55Z","timestamp":1790240275807,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T00:00:00Z","timestamp":1606348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006342","name":"Association Vaincre la Mucoviscidose","doi-asserted-by":"publisher","award":["RF20150501432"],"award-info":[{"award-number":["RF20150501432"]}],"id":[{"id":"10.13039\/501100006342","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006342","name":"Association Vaincre la Mucoviscidose","doi-asserted-by":"publisher","award":["RIF20160501701"],"award-info":[{"award-number":["RIF20160501701"]}],"id":[{"id":"10.13039\/501100006342","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006342","name":"Association Vaincre la Mucoviscidose","doi-asserted-by":"publisher","award":["RIF20170502058"],"award-info":[{"award-number":["RIF20170502058"]}],"id":[{"id":"10.13039\/501100006342","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT148593"],"award-info":[{"award-number":["PJT148593"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cystic fibrosis (CF), a genetic disorder, is characterized by chronic lung disease. Small non\u2010coding RNAs are key regulators of gene expression and participate in various processes, which are dysregulated in CF; however, they remain poorly studied. Here, we determined the complete microRNAs (miRNAs) expression pattern in three CF <jats:italic>ex vivo<\/jats:italic> models. The miRNA profiles of air\u2013liquid interface cultures of airway epithelia (bronchi, nasal cells, and nasal polyps) samples from patients with CF and non\u2010CF controls were obtained by deep sequencing. Compared with non\u2010CF controls, several miRNAs were deregulated in CF samples; for instance, miR\u2010181a\u20105p and the miR\u2010449 family were upregulated. Moreover, mature miRNAs often showed variations (i.e. isomiRs) relative to their reference sequence, such as miR\u2010101, suggesting that miRNAs consist of heterogeneous repertoires of multiple isoforms with different effects on gene expression. Analysis of miR\u2010181a\u20105p and miR\u2010101\u20103p roles indicated that they regulate the expression of WISP1, a key component of cell proliferation\/migration programs. We showed that miR\u2010101 and miR\u2010181a\u20105p participated in aberrant recapitulation of wound healing programs by controlling WISP1 mRNA and protein level. Our miRNA expression data bring new insights into CF physiopathology and define new potential therapeutic targets in CF. \u00a9 2020 The Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.5571","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T14:01:31Z","timestamp":1603461691000},"page":"186-197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<scp>miRNA<\/scp> repertoires of cystic fibrosis <i>ex vivo<\/i> models highlight <scp>miR\u2010181a<\/scp> and <scp>miR<\/scp>\u2010101 that regulate <scp>WISP1<\/scp> expression"],"prefix":"10.1002","volume":"253","author":[{"given":"Alexandra","family":"Pommier","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Montpellier, Laboratoire de G\u00e9n\u00e9tique de Maladies Rares EA7402  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Varilh","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Montpellier, Laboratoire de G\u00e9n\u00e9tique de Maladies Rares EA7402  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solenne","family":"Bleuse","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Montpellier, Laboratoire de G\u00e9n\u00e9tique de Maladies Rares EA7402  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Del\u00e9tang","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Montpellier, Laboratoire de G\u00e9n\u00e9tique de Maladies Rares EA7402  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Bonini","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Montpellier, Laboratoire de G\u00e9n\u00e9tique de Maladies Rares EA7402  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Bergougnoux","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Montpellier, Laboratoire de G\u00e9n\u00e9tique de Maladies Rares EA7402  Montpellier France"},{"name":"CHU de Montpellier, Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8182-943X","authenticated-orcid":false,"given":"Emmanuelle","family":"Brochiero","sequence":"additional","affiliation":[{"name":"Centre de Recherche du Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)  Montr\u00e9al QC Canada"},{"name":"D\u00e9partement de M\u00e9decine Universit\u00e9 de Montr\u00e9al  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Koenig","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Montpellier, Laboratoire de G\u00e9n\u00e9tique de Maladies Rares EA7402  Montpellier France"},{"name":"CHU de Montpellier, Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireille","family":"Claustres","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Montpellier, Laboratoire de G\u00e9n\u00e9tique de Maladies Rares EA7402  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0059-9394","authenticated-orcid":false,"given":"Magali","family":"Taulan\u2010Cadars","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Montpellier, Laboratoire de G\u00e9n\u00e9tique de Maladies Rares EA7402  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202960"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40348-014-0012-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07415"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.06.017"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2014.04.039"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiolchem.2010.06.001"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2225110"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2012.07.005"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.198390"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902669"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2012-0430OC"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00076214"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7221"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00113214"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2018.03.010"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2478\/mngs-2014-0001"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbt086"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1015281107"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2018.01.004"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a008078"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12595"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2011-200089"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-104"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055214"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni178"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112010"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-2570-2"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2007.11.007"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2007.09.009"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00813-z"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2015.11.006"},{"key":"e_1_2_8_33_1","first-page":"346","article-title":"The NF\u2010\u03baB signaling in cystic fibrosis lung disease: pathophysiology and therapeutic potential","volume":"9","author":"Bodas M","year":"2010","journal-title":"Discov Med"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-9-16"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1210906109"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00221711"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-4-8"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200410-1398OC"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.205146.116"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.10.17.17011"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050837"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.4103\/jrms.JRMS_1054_17"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04733"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122077"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-016-0346-6"}],"container-title":["The Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.5571","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/path.5571","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/path.5571","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:33:09Z","timestamp":1761057189000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/path.5571"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,26]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/path.5571"],"URL":"https:\/\/doi.org\/10.1002\/path.5571","archive":["Portico"],"relation":{},"ISSN":["0022-3417","1096-9896"],"issn-type":[{"value":"0022-3417","type":"print"},{"value":"1096-9896","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,26]]},"assertion":[{"value":"2019-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}