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As tumour sequencing scales towards clinical implementation, practical difficulties in scheduling and obtaining fresh tissue biopsies at scale, including blood samples as surrogates for matched \u2018normal\u2019 DNA, have focused attention on the use of formalin\u2010preserved clinical samples collected routinely for diagnostic purposes. In practice, such samples often contain both tumour and normal cells which, if correctly partitioned, could be used to profile both tumour and normal genomes, thus identifying somatic alterations. Here we report a semi\u2010automated method for laser microdissecting entire slide\u2010mounted tissue sections to enrich for cells of interest with sufficient yield for whole genome and transcriptome sequencing. Using this method, we demonstrated enrichment of tumour material from mixed tumour\u2013normal samples by up to 67%. Leveraging new methods that allow for the extraction of high\u2010quality nucleic acids from small amounts of formalin\u2010fixed tissues, we further showed that the method was successful in yielding sequence data of sufficient quality for use in BC Cancer's Personalized OncoGenomics (POG) program. \u00a9 2020 The Pathological Society of Great Britain and Ireland. 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