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By tracking and phenotypic profiling of LD<jats:sup>hi<\/jats:sup> cells, we find that LD<jats:sup>hi<\/jats:sup>CD19<jats:sup>+<\/jats:sup>, LD<jats:sup>hi<\/jats:sup>CD11b<jats:sup>+<\/jats:sup>, and LD<jats:sup>hi<\/jats:sup>Ly6G<jats:sup>+<\/jats:sup> immune cell populations appear in the spleen, thymus, and tumour tissues in a syngeneic tumour model. Using a contact\u2010dependent reporter system, we discover a LD<jats:sup>hi<\/jats:sup>CCR7<jats:sup>hi<\/jats:sup> immunosuppressive cell population that migrates from tumour tissues to the spleen and thymus. Hence, we engineered a family of chimeric antigen receptor\u2010modified macrophages (CAR\u2010Ms) that direct macrophages to CCR7\u2010positive cells and show that the cytosolic domain from Mer receptor tyrosine kinase (MerTK) triggers tumour cell cytotoxicity by the CAR\u2010Ms. <jats:italic>In vivo<\/jats:italic>, CCR7\u2010targeted CAR\u2010Ms suppressed tumour growth and prolonged survival by preventing metastasis and by inducing systemic anti\u2010tumour immunity through retarding the migration of LD<jats:sup>hi<\/jats:sup>CCR7<jats:sup>hi<\/jats:sup> immunosuppressive cells from tumour tissues to distal immune organs, indicating an important role for CCR7 in tumour cell\u2010induced immune tolerance. \u00a9 2020 The Pathological Society of Great Britain and Ireland. Published by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.5585","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T09:06:00Z","timestamp":1604394360000},"page":"247-257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":110,"title":["Chimeric antigen receptor\u2010modified macrophages trigger systemic anti\u2010tumour immunity"],"prefix":"10.1002","volume":"253","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5699-5324","authenticated-orcid":false,"given":"Zhiyuan","family":"Niu","sequence":"first","affiliation":[{"name":"Synthetic Biology Engineering Lab of Henan Province, School of Life Sciences and Technology Xinxiang Medical University  Xinxiang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanxu","family":"Chen","sequence":"additional","affiliation":[{"name":"Synthetic Biology Engineering Lab of Henan Province, School of Life Sciences and 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