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NETs constitute a defence mechanism to entrap and kill fungi and bacteria. Tumours induce the formation of NETs to the advantage of the malignancy via a variety of mechanisms shown in mouse models. Here, we investigated the presence of NETs in a variety of human solid tumours and their association with IL\u20108 (CXCL8) protein expression and CD8<jats:sup>+<\/jats:sup> T\u2010cell density in the tumour microenvironment. Multiplex immunofluorescence panels were developed to identify NETs in human cancer tissues by co\u2010staining with the granulocyte marker CD15, the neutrophil marker myeloperoxidase and citrullinated histone H3 (H3Cit), as well as IL\u20108 protein and CD8<jats:sup>+<\/jats:sup> T cells. Three ELISA methods to detect and quantify circulating NETs in serum were optimised and utilised. Whole tumour sections and tissue microarrays from patients with non\u2010small cell lung cancer (NSCLC; <jats:italic>n<\/jats:italic>\u00a0=\u200914), bladder cancer (<jats:italic>n<\/jats:italic>\u00a0=\u200914), melanoma (<jats:italic>n<\/jats:italic>\u00a0=\u200911), breast cancer (<jats:italic>n<\/jats:italic>\u00a0=\u200931), colorectal cancer (<jats:italic>n<\/jats:italic>\u00a0=\u200920) and mesothelioma (<jats:italic>n<\/jats:italic>\u00a0=\u200961) were studied. Also, serum samples collected retrospectively from patients with metastatic melanoma (<jats:italic>n<\/jats:italic>\u00a0=\u200912) and NSCLC (<jats:italic>n<\/jats:italic>\u00a0=\u200934) were ELISA assayed to quantify circulating NETs and IL\u20108. NETs were detected in six different human cancer types with wide individual variation in terms of tissue density and distribution. At least in NSCLC, bladder cancer and metastatic melanoma, NET density positively correlated with IL\u20108 protein expression and inversely correlated with CD8<jats:sup>+<\/jats:sup> T\u2010cell densities. In a series of serum samples from melanoma and NSCLC patients, a positive correlation between circulating NETs and IL\u20108 was found. In conclusion, NETs are detectable in formalin\u2010fixed human biopsy samples from solid tumours and in the circulation of cancer patients with a considerable degree of individual variation. NETs show a positive association with IL\u20108 and a trend towards a negative association with CD8<jats:sup>+<\/jats:sup> tumour\u2010infiltrating lymphocytes. \u00a9 2021 The Authors. <jats:italic>The Journal of Pathology<\/jats:italic> published by John Wiley &amp; Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland.<\/jats:p>","DOI":"10.1002\/path.5753","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T06:39:20Z","timestamp":1624948760000},"page":"190-201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":103,"title":["Heterogenous presence of neutrophil extracellular traps in human solid tumours is partially dependent on <scp>IL<\/scp>\u20108"],"prefix":"10.1002","volume":"255","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7628-8050","authenticated-orcid":false,"given":"Carlos E","family":"de Andrea","sequence":"first","affiliation":[{"name":"Department of Pathology Cl\u00ednica Universidad de Navarra  Pamplona Spain"},{"name":"Department of Anatomy, Physiology and Pathology University of Navarra  Pamplona Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer (CIBERONC)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Carmen","family":"Ochoa","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer (CIBERONC)  Madrid Spain"},{"name":"Division of Immunology and Immunotherapy, Center for Applied Medical Research (CIMA) University of Navarra  Pamplona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Villalba\u2010Esparza","sequence":"additional","affiliation":[{"name":"Department of Pathology Cl\u00ednica Universidad de Navarra  Pamplona Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer (CIBERONC)  Madrid 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