{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T05:27:28Z","timestamp":1785389248760,"version":"3.55.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T00:00:00Z","timestamp":1646265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015570","name":"Blood Cancer UK","doi-asserted-by":"publisher","award":["13006"],"award-info":[{"award-number":["13006"]}],"id":[{"id":"10.13039\/501100015570","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015570","name":"Blood Cancer UK","doi-asserted-by":"publisher","award":["19010"],"award-info":[{"award-number":["19010"]}],"id":[{"id":"10.13039\/501100015570","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"publisher","award":["C8333\/A29707"],"award-info":[{"award-number":["C8333\/A29707"]}],"id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>In situ<\/jats:italic>\n                    follicular neoplasia (ISFN) is usually an occasional incidental finding in lymph nodes by BCL2 immunohistochemistry, and its true scale is unknown. We have identified six cases of follicular lymphoma (FL) with a history of solid neoplasm 4\u201316\u2009years ago, from which ISFN was identified widely in the surgically cleared lymph nodes (LNs). Using clone\u2010specific PCR and BaseScope\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridisation with primers or probes specific to the\n                    <jats:italic>VDJ<\/jats:italic>\n                    or\n                    <jats:italic>BCL2\u2013IGHJ<\/jats:italic>\n                    junction sequence, we confirmed the clonal identity among different ISFNs and overt\u2010FL in each of the four cases successfully investigated. Mutation analyses of overt\u2010FL by targeted next\u2010generation sequencing identified multiple potential pathogenic changes involving\n                    <jats:italic>CREBBP<\/jats:italic>\n                    ,\n                    <jats:italic>EZH2<\/jats:italic>\n                    ,\n                    <jats:italic>KMT2D<\/jats:italic>\n                    ,\n                    <jats:italic>TNFRS14<\/jats:italic>\n                    , and\n                    <jats:italic>STAT6<\/jats:italic>\n                    . Further investigations of these mutations in paired ISFNs using Fluidigm PCR and Illumina sequencing showed the presence of the FL\u2010associated mutations in early lesions for two of the six cases investigated (\n                    <jats:italic>CREBBP<\/jats:italic>\n                    and\n                    <jats:italic>KMT2D<\/jats:italic>\n                    in one case and\n                    <jats:italic>STAT6<\/jats:italic>\n                    in the other), with one case displaying stepwise accumulation of its observed mutations. Remarkably, there were considerable divergences in\n                    <jats:italic>BCL2<\/jats:italic>\n                    variants among different ISFN\u2010involved lymph nodes in all four cases successfully investigated, indicating ongoing intraclonal diversification by somatic hypermutation machinery. Our findings demonstrate widespread distribution of ISFN lesions, further implicating their dynamic nature with the neoplastic cells undergoing active trafficking and clonal evolution. \u00a9 2021 The Authors.\n                    <jats:italic>The Journal of Pathology<\/jats:italic>\n                    published by John Wiley &amp; Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.\n                  <\/jats:p>","DOI":"10.1002\/path.5861","type":"journal-article","created":{"date-parts":[[2021,12,26]],"date-time":"2021-12-26T23:48:41Z","timestamp":1640562521000},"page":"369-377","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Widespread\n                    <i>in situ<\/i>\n                    follicular neoplasia in patients who subsequently developed follicular lymphoma"],"prefix":"10.1002","volume":"256","author":[{"given":"Rachel","family":"Dobson","sequence":"first","affiliation":[{"name":"Division of Cellular and Molecular Pathology, Department of Pathology University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Wotherspoon","sequence":"additional","affiliation":[{"name":"Histopathology Department The Royal Marsden Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizhang Alexander","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Cellular and Molecular Pathology, Department of Pathology University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Cucco","sequence":"additional","affiliation":[{"name":"Division of Cellular and Molecular Pathology, Department of Pathology University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Cellular and Molecular Pathology, Department of Pathology University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Tang","sequence":"additional","affiliation":[{"name":"Division of Cellular and Molecular Pathology, Department of Pathology University of Cambridge  Cambridge UK"},{"name":"Department of Pathology, West China Hospital Sichuan University  Chengdu PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1017-5045","authenticated-orcid":false,"given":"Ming\u2010Qing","family":"Du","sequence":"additional","affiliation":[{"name":"Division of Cellular and Molecular Pathology, Department of Pathology University of Cambridge  Cambridge UK"},{"name":"Department of Histopathology, Addenbrooke\u2019s Hospital Cambridge University 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