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Direct experimental evidence clarifying the role of intestinal mucosa attributes in IgAN is lacking. In this study, a mouse model was established via multiple low\u2010dose intraperitoneal injections of\n                    <jats:italic>Lactobacillus casei<\/jats:italic>\n                    cell wall extract (LCWE) emulsified with Complete Freund's Adjuvant (CFA). We found continuous and stable deposition of IgA in glomerular mesangial areas, accompanying high circulating levels of IgA and IgA\u2013IgG complexes. Expression of the key extracellular matrix components collagen IV and fibronectin also increased in the mesangial areas of LCWE\u2010induced mice. IgA\n                    <jats:sup>+<\/jats:sup>\n                    B220\n                    <jats:sup>+<\/jats:sup>\n                    B\u2010cell proportion increased in the small intestine (SI), Peyer's patches, inguinal lymph nodes, spleen, and bone marrow. The intestinal barrier was dysfunctional in the LCWE\u2010induced mice, and consistent with this, higher levels of serum zonulin (namely prehaptoglobin\u20102), a regulator of epithelial and endothelial barrier function, were observed in patients with IgAN. Hematoxylin and eosin staining results indicated that immune tissues such as liver, spleen, and lymph nodes showed an inflammatory response and focal lesions. Glucocorticoid methylprednisolone treatment could alleviate serum IgA and IgA\u2013IgG complex levels and mesangial IgA deposition. Taken together, our results indicate that we have successfully constructed a mouse model with IgA deposition in the mesangial areas of the glomeruli and provide evidence for the connection between the intestinal barrier and elevated circulating IgA and IgA\u2013IgG in IgAN. \u00a9 2022 The Pathological Society of Great Britain and Ireland.\n                  <\/jats:p>","DOI":"10.1002\/path.5884","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T06:37:31Z","timestamp":1644993451000},"page":"262-273","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Sustained release of\n                    <i>Lactobacillus casei<\/i>\n                    cell wall extract can induce a continuous and stable\n                    <scp>IgA<\/scp>\n                    deposition 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