{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:52:24Z","timestamp":1786978344921,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T00:00:00Z","timestamp":1674864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672821"],"award-info":[{"award-number":["81672821"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81773101"],"award-info":[{"award-number":["81773101"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81802306"],"award-info":[{"award-number":["81802306"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872041"],"award-info":[{"award-number":["81872041"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81903002"],"award-info":[{"award-number":["81903002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    For stage III colorectal cancer (CRC) patients with a high risk of recurrence, intensified adjuvant chemotherapy can improve overall survival. We aimed to develop a circulating tumor DNA (ctDNA) methylation marker model for predicting the relapse risk of stage III CRC patients. Differentially methylated markers identified between 53 normal mucosa samples and 165 CRC tissue samples, as well as between plasma samples from 75 stage I\/II (early\u2010stage) CRC patients and 55 stage IV (late\u2010stage) CRC patients, were analyzed using Student's\n                    <jats:italic>t<\/jats:italic>\n                    \u2010tests. The overlapping methylation markers shared by plasma and tissue samples were used to establish a methylation marker model to evaluate the tumor burden in the peripheral blood of CRC patients using the random forest method. This model was verified in the validation cohort (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200944) and then applied to predict recurrence risk in 50 stage III CRC patients and monitor the clinical disease course in serial samples from four CRC patients. We built a five\u2010marker\u2010based ctDNA methylation model that had high sensitivity (84.21%) and specificity (84%) in identifying late\u2010stage CRC in a validation cohort containing 24 stage I\/II CRC patients and 20 stage IV CRC patients. The model achieved high sensitivity (87.5%) and specificity (94.12%) in predicting tumor relapse in an independent cohort of 50 stage III CRC patients and could be an independent recurrence risk factor for stage III patients [Hazard ratio (HR), 60.4; 95% confidence interval (CI): 7.68\u2013397;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20099.73e\u22125]. Analysis of serial blood samples of CRC showed that the model could monitor disease relapse earlier than imaging examination and serum carcinoembryonic antigen (CEA) and so may provide an opportunity for the early adjustment of therapeutic strategies. Moreover, the model could potentially monitor the clinical course and treatment response dynamically. \u00a9 2022 The Authors.\n                    <jats:italic>The Journal of Pathology<\/jats:italic>\n                    published by John Wiley &amp; Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.\n                  <\/jats:p>","DOI":"10.1002\/path.6047","type":"journal-article","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T06:19:47Z","timestamp":1672121987000},"page":"376-387","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Recurrence risk assessment for stage\n                    <scp>III<\/scp>\n                    colorectal cancer based on five methylation biomarkers in plasma cell\u2010free\n                    <scp>DNA<\/scp>"],"prefix":"10.1002","volume":"259","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology, Nanfang Hospital and Basic Medical College Southern Medical University  Guangzhou PR China"},{"name":"Department of Pathology, General Hospital of Southern Theater Command People's Liberation Army of China  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Nanfang Hospital and Basic Medical College Southern Medical University  Guangzhou PR China"},{"name":"Guangdong Province Key Laboratory of Molecular Tumor Pathology  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6584-4923","authenticated-orcid":false,"given":"Xuecong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, School of Basic Medicine Southern Medical University  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyong","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Urology, Nanfang Hospital Southern Medical University  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicheng","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Pathology, Nanfang Hospital and Basic Medical College Southern Medical University  Guangzhou PR China"},{"name":"Guangdong Province Key Laboratory of Molecular Tumor Pathology  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoliang","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Nanfang Hospital Southern Medical University  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhi","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Pathology, General Hospital of Southern Theater Command People's Liberation Army of China  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Nanfang Hospital and Basic Medical College Southern Medical University  Guangzhou PR China"},{"name":"Guangdong Province Key Laboratory of Molecular Tumor Pathology  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaowan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Nanfang Hospital and Basic Medical College Southern Medical University  Guangzhou PR China"},{"name":"Guangdong Province Key Laboratory of Molecular Tumor Pathology  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Oncology, General Hospital of Southern Theater Command People's Liberation Army of China  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3911-5994","authenticated-orcid":false,"given":"Xuehui","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Nanfang Hospital and Basic Medical College Southern Medical University  Guangzhou PR China"},{"name":"Guangdong Province Key Laboratory of Molecular Tumor Pathology  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijun","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Nanfang Hospital Southern Medical University  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Li","sequence":"additional","affiliation":[{"name":"AnchorDx Medical Co., Ltd.  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