{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T03:18:27Z","timestamp":1785208707627,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017268","name":"Berlin Institute of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The origin of mucinous cystic neoplasms (MCNs) remains a major challenge in hepato\u2010pancreato\u2010biliary pathology. These cystic tumors are defined by their mucinous epithelium and ovarian\u2010like stroma, with an estimated 10% risk of progression to invasive carcinoma. The origin of the ovarian\u2010like stroma remains a subject of debate. In this study, we conducted immunohistochemical profiling, targeted DNA sequencing, and genome\u2010wide DNA methylation analysis on a cohort of 15 pancreatic MCNs (MCN\u2010P) and six hepatic MCNs (MCN\u2010L). Using immunohistochemistry and targeted DNA sequencing, we unequivocally established the diagnosis of MCN. Unsupervised DNA methylation profile analysis of reference classes of pancreatic neoplasms (11 entities and normal pancreatic tissue from 224 unique samples) revealed that MCN\u2010P predominantly forms a distinct group. In the DNA methylation landscape of liver tumors, encompassing five tumor types and normal bile duct tissue from 136 unique samples, MCN\u2010L demonstrated a specific methylation profile when compared with all other entities. Furthermore, within the DNA methylation landscape of ovarian tumors \u2013 featuring five tumor types, normal Fallopian tube, and normal ovarian tissue from 90 unique samples \u2013 we found that both MCN\u2010P and MCN\u2010L grouped with mucinous ovarian carcinoma and mucinous borderline ovarian tumors (mBOTs). Notably, low\u2010grade MCNs exhibited greater DNA methylation similarities to mBOTs, while high\u2010grade or invasive MCNs were primarily associated with mucinous ovarian carcinomas. When analyzing all samples together (19 tumor types and four normal tissue types,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009430), MCNs similarly grouped with mucinous ovarian tumors and normal ovarian tissue. Additionally, in a network analysis of differentially methylated probes, MCN\u2010P and MCN\u2010L share significant methylation traits, closely resembling mucinous ovarian tumors. In conclusion, our findings highlight that MCN\u2010P and MCN\u2010L are distinct entities in the landscape of pancreatic and hepatic tumors and show DNA methylation profile similarities with mucinous ovarian tumors, suggesting a potential common origin. \u00a9 2025 The Author(s).\n                    <jats:italic>The Journal of Pathology<\/jats:italic>\n                    published by John Wiley &amp; Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.\n                  <\/jats:p>","DOI":"10.1002\/path.6439","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T06:14:07Z","timestamp":1750832047000},"page":"10-24","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mucinous cystic neoplasms of the pancreas and liver share a similar\n                    <scp>DNA<\/scp>\n                    methylation profile with mucinous ovarian tumors"],"prefix":"10.1002","volume":"267","author":[{"given":"Zoe","family":"Leoni","sequence":"first","affiliation":[{"name":"Institute of Pathology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2972-2093","authenticated-orcid":false,"given":"Teodor G","family":"Calina","sequence":"additional","affiliation":[{"name":"Faculty of Physics Babe\u0219\u2010Bolyai University  Cluj\u2010Napoca Romania"},{"name":"TGC Ventures UG  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Janik","sequence":"additional","affiliation":[{"name":"German Cancer Consortium (DKTK) Partner Site Berlin, and German Cancer Research Center (DKFZ)  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Grafenhorst","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3041-694X","authenticated-orcid":false,"given":"Eliane T","family":"Taube","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher CM","family":"Neumann","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Tumor Immunology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BaoQing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer Sun Yat\u2010sen University Cancer Center  Guangzhou PR China"},{"name":"Guangdong Esophageal Cancer Research Institute  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena I","family":"Braicu","sequence":"additional","affiliation":[{"name":"Department of Gynaecology, European Competence Center for Ovarian Cancer Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"North Eastern German Society for Gynecological Cancer Tumor Bank Ovarian Cancer Network  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jalid","family":"Sehouli","sequence":"additional","affiliation":[{"name":"Department of Gynaecology, European Competence Center for Ovarian Cancer Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"North Eastern German Society for Gynecological Cancer Tumor Bank Ovarian Cancer Network  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Malinka","sequence":"additional","affiliation":[{"name":"Department of Surgery, Experimental Surgery, CCM, CVK, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzel","family":"Sch\u00f6ning","sequence":"additional","affiliation":[{"name":"Department of Surgery, Experimental Surgery, CCM, CVK, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johann","family":"Pratschke","sequence":"additional","affiliation":[{"name":"Department of Surgery, Experimental Surgery, CCM, CVK, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George A","family":"Calin","sequence":"additional","affiliation":[{"name":"Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center  Houston TX USA"},{"name":"The Non\u2010coding RNA Center The University of Texas MD Anderson Cancer Center  Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S","family":"Klimstra","sequence":"additional","affiliation":[{"name":"Department of Pathology Yale School of Medicine  New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamal K","family":"Benhamida","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Memorial Sloan Kettering Cancer Center  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Esposito","sequence":"additional","affiliation":[{"name":"Institute of Pathology Heinrich\u2010Heine\u2010University and University Hospital of D\u00fcsseldorf  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"M\u00f6bs","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Charit\u00e9 \u2013 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