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However, this may not always be the case, and it is important to determine how often such recurrences represent new tumours. Ipsilateral primary\u2013recurrence pairs (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200978) were sequenced to test their clonal relatedness. Shared genetic events were identified from whole exome sequencing (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200954 pairs) using haplotype\u2010specific copy number and phylogenetic analysis. The remaining pairs were sequenced using a targeted panel or low\u2010coverage whole genome sequencing. We included 32 non\u2010recurrent DCIS to compare recurrent and non\u2010recurrent disease. We found that 7% of DCIS recurrences were non\u2010clonal by whole exome sequencing, indicative of a new breast carcinoma. Lower resolution methods detected a higher non\u2010clonality rate (29%). By comparing primary DCIS with their recurrence, we found that the evolution of DCIS to invasive disease was associated with increased ploidy and copy number events.\n                    <jats:italic>TP53<\/jats:italic>\n                    mutations were enriched in DCIS with clonal recurrence compared with non\u2010recurrent DCIS. Our results verify that\n                    <jats:italic>de novo<\/jats:italic>\n                    \u2018recurrent tumours\u2019 of independent origin occur in patients who may be at high risk. \u00a9 2025 The Author(s).\n                    <jats:italic>The Journal of Pathology<\/jats:italic>\n                    published by John Wiley &amp; Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.\n                  <\/jats:p>","DOI":"10.1002\/path.6461","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T15:30:53Z","timestamp":1761838253000},"page":"1-12","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phylogenetic analysis of paired breast carcinomas identifies genetic events associated with clonal recurrence and invasive 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