{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:52:11Z","timestamp":1789487531921,"version":"build-2803163510"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K08 AA029182"],"award-info":[{"award-number":["K08 AA029182"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30 CA016359"],"award-info":[{"award-number":["P30 CA016359"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30 DK034989"],"award-info":[{"award-number":["P30 DK034989"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Platelet\u2010derived microparticles (PMPs) constitute the majority of circulating microparticles in blood. PMPs carry cargo including RNA, protein, and miRNA, and play key pathophysiologic intercellular signaling roles in cardiovascular, autoimmune, and liver diseases. Most methods use fresh platelets treated with an agonist to generate PMPs, which has restricted the size and feasibility of human studies. With the rise of large biobanks, such as the UK Biobank (\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.ukbiobank.ac.uk\/\">https:\/\/www.ukbiobank.ac.uk\/<\/jats:ext-link>\n                    ), and because microparticles are retained in cryopreserved plasma, this study aimed to develop an efficient and reproducible method to isolate PMPs from banked cryopreserved human plasma to investigate their communication with target cells. We employed flow cytometry using 180\u20131,300\u2009nm size calibration beads and the platelet\u2010specific marker CD41 to identify and sort PMPs based on size and CD41 positivity. Following isolation, microparticle size and morphology were validated by electron microscopy and nanoparticle tracking analysis. To assess the utility of the microparticles for studies of cell\u2013cell interactions, we visualized microparticle uptake into human umbilical vein endothelial cells and THP\u20101 cells. Our method enables the isolation and downstream analysis of human PMPs in cell\u2013cell interactions, facilitating translational studies in large populations and rare diseases. \u00a9 2026 The Pathological Society of Great Britain and Ireland.\n                  <\/jats:p>","DOI":"10.1002\/path.70097","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:00:11Z","timestamp":1784034011000},"page":"131-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Applying flow cytometry\u2010based isolation of platelet\u2010derived microparticles to banked human plasma for cellular studies"],"prefix":"10.1002","volume":"270","author":[{"given":"Nima","family":"Fattahi","sequence":"first","affiliation":[{"name":"Section of Digestive Diseases, Department of Internal Medicine Yale University School of Medicine  New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarun","family":"Tyagi","sequence":"additional","affiliation":[{"name":"Section of Cardiovascular Medicine, Department of Internal Medicine Yale University School of Medicine  New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fallyn","family":"Kirlin","sequence":"additional","affiliation":[{"name":"Section of Digestive Diseases, Department of Internal Medicine Yale University School of Medicine  New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea De La Lama","family":"Flores","sequence":"additional","affiliation":[{"name":"Section of Digestive Diseases, Department of Internal Medicine Yale University School of Medicine  New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Hwa","sequence":"additional","affiliation":[{"name":"Section of Cardiovascular Medicine, Department of Internal Medicine Yale University School of Medicine  New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Iwakiri","sequence":"additional","affiliation":[{"name":"Section of Digestive Diseases, Department of Internal Medicine Yale University School of Medicine  New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8522-0381","authenticated-orcid":false,"given":"Matthew J","family":"McConnell","sequence":"additional","affiliation":[{"name":"Section of Digestive Diseases, Department of Internal Medicine Yale University School of Medicine  New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1967.tb08741.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44161-022-00108-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12882-019-1261-x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2019.11.001"},{"key":"e_1_2_7_6_1","first-page":"2916","article-title":"Extraction and identification of platelet\u2010derived microparticles","volume":"20","author":"Guo J","year":"2019","journal-title":"Mol Med Rep"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28814"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1597603"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2020.1743139"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","unstructured":"GrafI Salviano\u2010SilvaA BehrendsJ et al.High\u2010purity enrichment of extracellular vesicles from diverse sources by conventional and image\u2010based fluorescence activated cell sorters for robust downstream applications.bioRxiv2025; 10.12.681826. 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