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Yet in colorectal cancer (CRC), biomarkers and mechanisms that specify leader cells remain undefined. Here we identify biomarkers of leader cells and elucidate how they drive CRC collective invasion and metastasis. Using a 3D photoconvertible CRC spheroid model, we isolated leader and follower cells. Transcriptomic, functional and\n                    <jats:italic>in vivo<\/jats:italic>\n                    assays show that GLUT1, PLOD2, and CCL5 in leader cells are indispensable for collective invasion. CCL5 activates PI3K\/AKT signaling to up\u2010regulate GLUT1 and PLOD2 in leader cells. Moreover, GLUT1 and CCL5 could serve as specific biomarkers for leader cells in CRC collective invasion, and their co\u2010expression correlates with poor prognosis. Pharmacologic inhibition of GLUT1 and CCL5\u2010CCR5 impairs collective invasion. CCL5 and GLUT1 may define the leader cells and are required for CRC collective invasion as potential key regulators of metabolic shifts and collagen deposition. \u00a9 2026 The Author(s).\n                    <jats:italic>The Journal of Pathology<\/jats:italic>\n                    published by John Wiley &amp; Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.\n                  <\/jats:p>","DOI":"10.1002\/path.70100","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T09:19:16Z","timestamp":1786526356000},"page":"288-302","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>CCL5<\/scp>\n                    and\n                    <scp>GLUT1<\/scp>\n                    define leader cells in collective invasion of colorectal cancer"],"prefix":"10.1002","volume":"270","author":[{"given":"Zhaowen","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pathology, Nanfang Hospital, School of Basic Medical Sciences Southern Medical University  Guangzhou PR China"},{"name":"Guangdong Province Key Laboratory of Molecular Tumor Pathology  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaying","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Nanfang Hospital, School of Basic Medical Sciences Southern Medical University  Guangzhou PR China"},{"name":"Guangdong Province Key Laboratory of Molecular Tumor Pathology  Guangzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicheng","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Fifth Affiliated Hospital Sun Yat\u2010sen University  Zhuhai PR 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