{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:07:26Z","timestamp":1790705246064,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,8,29]],"date-time":"2001-08-29T00:00:00Z","timestamp":999043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The aim of this study was to characterize the activation of caspase\u20103 along the crypt\/villus axis in the normal and irradiated intestine and to compare active caspase\u20103 expression with existing apoptosis detection techniques. Small and large intestine were removed from mice at various time points after exposure to 8\u2009Gy \u03b3\u2010radiation. Positive apoptotic cells stained with an antibody against active caspase\u20103, haematoxylin and eosin (H&amp;E) or TUNEL were scored in histological sections of small and large intestinal crypts and villi. In the control intestine, active caspase\u20103 expression was rarely observed; however, expression was markedly increased following exposure to radiation and was predominantly confined to apoptotic bodies. Measurement of apoptosis in intestinal crypts using active caspase\u20103 expression gave similar results to apoptosis detected from H&amp;E\u2010stained sections. In the normal villus, active caspase\u20103 expression was observed infrequently and did not significantly increase following radiation, consistent with a lack of apoptotic body formation from H&amp;E sections. This study indicates that caspase\u20103 is activated in intestinal crypts but not in villi following \u03b3\u2010radiation. Active caspase\u20103 detection compared favourably with existing immunological techniques, suggesting that it is a suitable alternative method for apoptosis quantification. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/path.967","type":"journal-article","created":{"date-parts":[[2005,1,23]],"date-time":"2005-01-23T20:49:37Z","timestamp":1106513377000},"page":"285-292","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":126,"title":["Caspase activation during spontaneous and radiation\u2010induced apoptosis in the murine intestine"],"prefix":"10.1002","volume":"195","author":[{"given":"Emma","family":"Marshman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penelope D.","family":"Ottewell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher S.","family":"Potten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alastair J. 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