{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:31:00Z","timestamp":1791174660052,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,15]],"date-time":"2005-12-15T00:00:00Z","timestamp":1134604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>T\u2010cell acute lymphoblastic leukemia (T\u2010ALL) and T\u2010cell lymphoblastic lymphoma (T\u2010LL) and are often thought to represent a spectrum of a single disease. The malignant cells in T\u2010ALL and T\u2010LL are morphologically indistinguishable, and they share the expression of common cell surface antigens and cytogenetic characteristics. However, despite these similarities, differences in the clinical behavior of T\u2010ALL and T\u2010LL are observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>We analyzed the gene expression profiles of T\u2010ALL and T\u2010LL samples obtained from Children's Oncology Group (COG) tumor banks using DNA arrays. Immunohistochemistry was also performed to validate the expression of selected targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Unsupervised hierarchical clustering of all samples showed complete segregation of T\u2010ALL and T\u2010LL into distinct clusters. Next, we identified the top 201 genes that best differentiated T\u2010ALL from T\u2010LL using significance analysis of microarrays (SAM), a supervised statistical approach. Genes representing several functional groups were differentially expressed in T\u2010LL and T\u2010ALL. Prediction analysis of microarrays (PAM) identified a subset of genes, which accurately classified all 19 T\u2010ALL and T\u2010LL samples with an overall misclassification error rate of 0. Immunohistochemical validation of protein expression of selected genes identified by microarray analysis confirmed overexpression of MLL\u20101 in T\u2010LL tumor cells compared to T\u2010ALL and CD47 in T\u2010ALL tumors cells when compared to T\u2010LL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Despite significant similarities between the malignant T\u2010cell precursors, clear differences in the gene expression profiles were observed between T\u2010ALL and T\u2010LL implying underlying differences in the biology of the two entities. Pediatr Blood Cancer \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.20550","type":"journal-article","created":{"date-parts":[[2005,12,15]],"date-time":"2005-12-15T18:52:28Z","timestamp":1134672748000},"page":"130-140","source":"Crossref","is-referenced-by-count":122,"title":["Gene expression profiling reveals intrinsic differences between T\u2010cell acute lymphoblastic leukemia and T\u2010cell lymphoblastic lymphoma"],"prefix":"10.1002","volume":"47","author":[{"given":"Elizabeth A.","family":"Raetz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherrie 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