{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:10:56Z","timestamp":1790863856932,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,8,1]],"date-time":"2006-08-01T00:00:00Z","timestamp":1154390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The Wilms Tumor gene (<jats:italic>WT1<\/jats:italic>) encodes a transcription factor involved in kidney development and malignancy. <jats:italic>WT1<\/jats:italic> expression in a subpopulation of early CD34+ cells has suggested its involvement in hematopoiesis. <jats:italic>WT1<\/jats:italic> is aberrantly expressed in leukemias. High expression of <jats:italic>WT1<\/jats:italic> at diagnosis has been associated with unfavorable prognosis in adult acute myeloid leukemia (AML). The prognostic relevance of <jats:italic>WT1<\/jats:italic> expression in pediatric AML was evaluated in only one study, including 47 patients, which showed that very low levels of <jats:italic>WT1<\/jats:italic> at presentation were associated with an excellent outcome. To test the validity of these findings we measured levels of <jats:italic>WT1<\/jats:italic> in 41 newly diagnosed pediatric AML of the non\u2010M3 FAB subtype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>Patients were treated according to an AML\u2010BFM 83\u2010based protocol in a single institution. Mononucleated cells obtained from presentation BM aspirates were cryopreserved and later thawed and used for total RNA extraction and cDNA synthesis. The quantitative assessment of <jats:italic>WT1<\/jats:italic> transcripts was made by real\u2010time PCR (RQ\u2010PCR). <jats:italic>WT1<\/jats:italic> transcripts values were normalized with respect to the number of <jats:italic>ABL<\/jats:italic> transcripts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>WT1<\/jats:italic> levels were significantly higher in patients bearing favorable chromosome abnormalities, t(8;21) and inv(16) (<jats:italic>P<\/jats:italic>\u2009=\u20090.002). Higher levels of <jats:italic>WT1<\/jats:italic> expression were unexpectedly associated with a higher probability of overall survival by Cox regression analysis (<jats:italic>P<\/jats:italic>\u2009=\u20090.002). Multivariate regression analysis could not discriminate between the effects of <jats:italic>WT1<\/jats:italic> and cytogenetics on survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Higher <jats:italic>WT1<\/jats:italic> expression was associated with favorable cytogenetics subtypes and accordingly with better outcome in children with AML in this study. Pediatr Blood Cancer 2007;49:133\u2013138. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.20953","type":"journal-article","created":{"date-parts":[[2006,8,2]],"date-time":"2006-08-02T18:20:29Z","timestamp":1154542829000},"page":"133-138","source":"Crossref","is-referenced-by-count":26,"title":["Prognostic significance of <i>WT1<\/i> gene expression in pediatric acute myeloid leukemia"],"prefix":"10.1002","volume":"49","author":[{"given":"Patricia C.","family":"Rodrigues","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e1tiro N.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos B.","family":"Viana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo I.","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre E.","family":"Nowill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia R.","family":"Brandalise","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A.","family":"Yunes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,6,5]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"97","article-title":"A novel tumor suppressor gene inactivated in Wilms' tumor","volume":"4","author":"Haber DA","year":"1992","journal-title":"New Biol"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90601-A"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.5131"},{"key":"e_1_2_6_5_2","first-page":"312","article-title":"Expression of the Wilms'tumor gene (WT1) in normal hemopoiesis","volume":"25","author":"Baird PN","year":"1997","journal-title":"Exp Hematol"},{"key":"e_1_2_6_6_2","first-page":"945","article-title":"The Wilms' tumor gene is expressed in a subset of CD34+ progenitors and downregulated early in course of differentiation in vitro","volume":"25","author":"Maurer U","year":"1997","journal-title":"Exp Hematol"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.9.3486"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"4230","DOI":"10.1182\/blood.V90.10.4230","article-title":"Wilms tumor gene (Wt1) mRNA is equally expressed in blast cells from acute myeloid leukemia and normal CD34+ progenitors","volume":"90","author":"Maurer U","year":"1997","journal-title":"Blood"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.8.1897"},{"key":"e_1_2_6_10_2","first-page":"1060","article-title":"Presence of the Wilms' tumor gene (WT1) transcripts and the WT1 nuclear protein in the majority of human acute leukemias","volume":"9","author":"Menssen HD","year":"1995","journal-title":"Leukemia"},{"key":"e_1_2_6_11_2","first-page":"405","article-title":"Expression of the Wilms' tumor gene (WT1) in human leukemias","volume":"6","author":"Miwa H","year":"1992","journal-title":"Leukemia"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.9.3071.3071"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.4.1405"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.6.2267.bloodjournal8862267"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428190009059281"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401341"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.9.3272"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.10.503"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V90.3.1217"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04552.x"},{"key":"e_1_2_6_21_2","first-page":"926","article-title":"Prognostic implications of Wilms' tumor gene (WT1) expression in patients with de novo acute myeloid leukemia","volume":"89","author":"Barragan E","year":"2004","journal-title":"Haematologica"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2400620"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403809"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1999.tb01121.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1159\/000040973"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1080\/088800199277434"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10024-001-0208-x"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402512"},{"key":"e_1_2_6_29_2","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1182\/blood.V75.10.1932.1932","article-title":"Identification of two risk groups in childhood acute myelogenous leukemia after therapy intensification in study AML\u2010BFM\u201083 as compared with study AML\u2010BFM\u201078. AML\u2010BFM Study Group","volume":"75","author":"Creutzig U","year":"1990","journal-title":"Blood"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1976.tb03563.x"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.2223\/JPED.1108"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.1.308.308"},{"key":"e_1_2_6_33_2","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1182\/blood.V88.1.377a.377a","article-title":"High frequency of acute promyelocytic leukemia among Latinos with acute myeloid leukemia","volume":"88","author":"Otero JC","year":"1996","journal-title":"Blood"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_6_35_2","first-page":"1984","article-title":"Multiplex PCR for quality control of template RNA\/cDNA in RT\u2010PCR assays","volume":"11","author":"Watzinger F","year":"1998","journal-title":"Leukemia"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401592"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403136"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402675"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminoncol.2003.12.029"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1080\/10428190410001693551"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1999.01304.x"}],"container-title":["Pediatric Blood &amp; Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpbc.20953","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pbc.20953","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T14:38:02Z","timestamp":1699972682000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pbc.20953"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,5]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["10.1002\/pbc.20953"],"URL":"https:\/\/doi.org\/10.1002\/pbc.20953","archive":["Portico"],"relation":{},"ISSN":["1545-5009","1545-5017"],"issn-type":[{"value":"1545-5009","type":"print"},{"value":"1545-5017","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,5]]}}}