{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T13:58:25Z","timestamp":1761919105338},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,4,24]],"date-time":"2008-04-24T00:00:00Z","timestamp":1208995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cancer\/testis antigens (CTA) represent a heterogeneous group of antigens expressed nearly exclusively in tumour cells and testis. Recently, we identified phospholipase A1 beta (a CTA also known as lipase member I, LIPI) as a gene with high expression in Ewing family tumours (EFT). In the present paper we analyzed expression of LIPI in a panel of normal tissues and tumour samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>The expression of CTA in EFT and normal tissues was analyzed by using DNA microarray datasets. Expression of LIPI in EFT, a panel of other tumour samples, and normal tissues was analyzed by using RT\u2010PCR and quantitative RT\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>LIPI was expressed in EFT samples but not in other investigated tumour samples. Expression of LIPI in normal tissues was restricted to testis and thyroid. However, expression in these tissues was low compared with EFT. Interestingly testis as well as thyroid expressed all analyzed EFT\u2010associated transcripts, suggesting that these tissues harbour a small cell population with molecular features of EFT. The sensitivity of the LIPI RT\u2010PCR was similar to the sensitivity of the conventional EWSR1\u2010FLI1 RT\u2010PCR, suggesting that LIPI might be useful as additional diagnostic target structure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The human cancer\/testis antigen LIPI is highly expressed in Ewing family tumours and can be easily detected by RT\u2010PCR or quantitative RT\u2010PCR. LIPI might be an interesting target for the development of future diagnostic tools or treatment strategies. Pediatr Blood Cancer 2008;51:228\u2013234. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.21602","type":"journal-article","created":{"date-parts":[[2008,4,24]],"date-time":"2008-04-24T14:57:34Z","timestamp":1209049054000},"page":"228-234","source":"Crossref","is-referenced-by-count":36,"title":["Membrane\u2010associated phospholipase A1 beta (LIPI) Is an Ewing tumour\u2010associated cancer\/testis antigen"],"prefix":"10.1002","volume":"51","author":[{"given":"Juergen L.","family":"Foell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Hesse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ines","family":"Volkmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin J.","family":"Schmiedel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingo","family":"Neumann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin S.","family":"Staege","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,4,24]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2005.08.007"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00043426-200105000-00009"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/89044"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-4059"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10276"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg124"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M213018200"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00740-07"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10048-006-0032-6"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2005.04.008"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2007.02.027"},{"key":"e_1_2_7_13_2","first-page":"1","article-title":"The cancer\/testis genes: Review, standardization, and commentary","volume":"4","author":"Scanlan MJ","year":"2004","journal-title":"Cancer Immun"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0108"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.1.190"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22118"},{"key":"e_1_2_7_18_2","first-page":"4752","article-title":"XAGE\u20101, a new gene that is frequently expressed in Ewing's sarcoma","volume":"60","author":"Liu XF","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.23.700"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1677\/ERC-06-0023"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3801"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2006.04.006"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24808"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1616208"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1476-5586(04)80047-2"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2006.01.013"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-04-014480"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20081"},{"key":"e_1_2_7_29_2","first-page":"1073","article-title":"The human lipid phosphate phosphatase\u20103 decreases the growth, survival, and tumorigenesis of ovarian cancer cells: Validation of the lysophosphatidic acid signaling cascade as a target for therapy in ovarian cancer","volume":"63","author":"Tanyi JL","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404787"}],"container-title":["Pediatric Blood &amp; 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