{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T07:04:01Z","timestamp":1779347041252,"version":"3.51.4"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,7,10]],"date-time":"2008-07-10T00:00:00Z","timestamp":1215648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>T\u2010lymphoblastic lymphoma (T\u2010LBL) accounts for 25\u201330% of childhood non\u2010Hodgkin's lymphoma and is closely related to T\u2010lymphoblastic leukemia (T\u2010ALL). Recently, we demonstrated distinct differences in gene expression between childhood T\u2010LBL and T\u2010ALL, but molecular pathogenesis and relevant protein expression patterns in T\u2010LBL remain poorly understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>Children with T\u2010LBL with disseminated disease were registered and treated on COG protocol 5971. Paraffin\u2010embedded tumor tissue was obtained at diagnosis for immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) studies. We determined the pattern and intensity of staining for c\u2010Myc, Skp2, Mib\u20101, p53, TCL\u20101, bcl\u20102, and bcl\u20106 proteins by IHC and c\u2010Myc, p53, bcl\u20102, bcl\u20106, and TCR \u03b1\/\u03b4 molecular alterations by FISH in 22 pediatric T\u2010LBL cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The majority of T\u2010LBL samples expressed Mib\u20101 (59%) and c\u2010Myc (77%) proteins in greater than 50% of the cells, but Skp2 (14%), p53 (14%), and bcl\u20102 (23%) expression was less common. FISH studies demonstrated 18% gains and 10% losses in c\u2010Myc, 16% gains in p53, 12% gains and 6% losses in bcl\u20102, and 6% gains and 19% losses in bcl\u20106 with little direct correlation between the IHC and FISH studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Childhood T\u2010LBL is a highly proliferative tumor associated with enhanced expression of c\u2010Myc protein, but without detectable c\u2010Myc molecular alterations. FISH studies did not identify consistent etiologies of molecular dysregulation, and future studies with other molecular approaches may be required to elucidate the molecular pathogenesis of childhood T\u2010LBL. Pediatr Blood Cancer 2008;51:489\u2013494. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.21666","type":"journal-article","created":{"date-parts":[[2008,7,10]],"date-time":"2008-07-10T13:42:34Z","timestamp":1215697354000},"page":"489-494","source":"Crossref","is-referenced-by-count":20,"title":["Characterization of childhood precursor T\u2010lymphoblastic lymphoma by immunophenotyping and fluorescent in situ hybridization: A report from the Children's Oncology Group"],"prefix":"10.1002","volume":"51","author":[{"given":"Kristi J.","family":"Smock","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilu","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheryl R.","family":"Tripp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warren G.","family":"Sanger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minnie","family":"Abromowitch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell S.","family":"Cairo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherrie L.","family":"Perkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,7,10]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"S3","article-title":"Advances in the diagnostic approach to childhood lymphoblastic malignant neoplasms","volume":"122","author":"Reddy KS","year":"2004","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_6_3_2","volume-title":"Non\u2010Hodgkin lymphoma in children, Cancer Medicine","author":"Cairo M","year":"2006"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.20342"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1995.13.6.1368"},{"key":"e_1_2_6_6_2","first-page":"#533","article-title":"Results of CCG\u20105941: Intensified multiagent chemotherapy and non\u2010cross resistant maintenance therapy for advanced lymphoblastic lymphoma in children and adolescents. Presented, American Society of Hematology Annual Meeting, 2006","volume":"108","author":"Abromowitch M","year":"2006","journal-title":"Blood"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404302"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.4.1270"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404243"},{"key":"e_1_2_6_10_2","first-page":"1116","article-title":"Genetic insights in the pathogenesis of T\u2010cell acute lymphoblastic leukemia","volume":"90","author":"De Keersmaecker K","year":"2005","journal-title":"Haematologica"},{"key":"e_1_2_6_11_2","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1182\/blood.V96.7.2543","article-title":"New recurring cytogenetic abnormalities and association of blast cell karyotypes with prognosis in childhood T\u2010cell acute lymphoblastic leukemia: A pediatric oncology group report of 343 cases","volume":"96","author":"Schneider NR","year":"2000","journal-title":"Blood"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2006.07.011"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.20550"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.05.047"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01091-06"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606108103"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1450406"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(98)00146-5"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/1042819029000611610"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V82.10.3151.3151"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.1.270"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1672"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.321004"}],"container-title":["Pediatric Blood &amp; Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpbc.21666","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pbc.21666","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T15:46:33Z","timestamp":1697643993000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pbc.21666"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,10]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["10.1002\/pbc.21666"],"URL":"https:\/\/doi.org\/10.1002\/pbc.21666","archive":["Portico"],"relation":{},"ISSN":["1545-5009","1545-5017"],"issn-type":[{"value":"1545-5009","type":"print"},{"value":"1545-5017","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,10]]}}}