{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:06:52Z","timestamp":1791094012763,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,11,12]],"date-time":"2008-11-12T00:00:00Z","timestamp":1226448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Despite overlapping features of T\u2010cell lymphoblastic lymphoma (T\u2010LLy) and T\u2010cell acute lymphoblastic leukemia (T\u2010ALL), which respond favorably to T\u2010ALL treatment, clinical and biological differences exist. We retrospectively assessed the prevalence of submicroscopic bone marrow (BM) minimal disseminated disease (MDD) at diagnosis and the early response to treatment (minimal residual disease\u2014MRD) and their prognostic significance in 17 children with stage III T\u2010LLy treated according to Berlin\u2010Frankfurt\u2010Munster (BFM) non\u2010Hodgkin lymphoma protocols.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>Four\u2010color flow cytometry (FC) was used for lymphoma associated immunophenotype and real\u2010time quantitative polymerase chain reaction (RQ\u2010PCR) for T\u2010cell receptor (TCR \u03b2\/\u03b4\/\u03b3) gene rearrangements with at least 0.01% sensitivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two markers per patient were identified in all cases using FC and in 80% using RQ\u2010PCR. BM MDD at diagnosis of \u22650.01% was detected by FC and RQ\u2010PCR in 88% and 80% of patients, respectively, and by at least one of the methods in all patients. A significant correlation was achieved between the methods by Pearson correlation analysis (<jats:italic>P<\/jats:italic>\u2009=\u20090.004). MRD levels significantly decreased to very low levels on day 33 in 9 out of 10 patients studied. The only patient that remained positive relapsed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MDD was prevalent in stage III T\u2010LLy, for which we could not prove a prognostic significance in the context of ALL\u2010like treatment. This study shows that both FC and RQ\u2010PCR methods are efficient for MDD and MRD analyses in T\u2010LLy. Pediatr Blood Cancer 2009;52:20\u201325. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.21823","type":"journal-article","created":{"date-parts":[[2008,11,13]],"date-time":"2008-11-13T02:42:18Z","timestamp":1226544138000},"page":"20-25","source":"Crossref","is-referenced-by-count":31,"title":["Bone marrow minimal disseminated disease (MDD) and minimal residual disease (MRD) in childhood T\u2010cell lymphoblastic lymphoma stage III, detected by flow cytometry (FC) and real\u2010time quantitative polymerase chain reaction (RQ\u2010PCR)"],"prefix":"10.1002","volume":"52","author":[{"given":"Batia","family":"Stark","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smadar","family":"Avigad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drorit","family":"Luria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigal","family":"Manor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tali","family":"Reshef\u2010Ronen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gali","family":"Avrahami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaac","family":"Yaniv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,11,12]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"725","volume-title":"Principles 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