{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:16:05Z","timestamp":1790291765934,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,2,11]],"date-time":"2009-02-11T00:00:00Z","timestamp":1234310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Multidrug resistance is a key factor for the sobering outcome of relapsed and metastatic human hepatoblastoma (HB). Gene directed treatment approaches were recently identified as possible treatment options against advanced HB, in which standard chemotherapy regimens are partially insufficient. The aim of this study was to systematically analyze the effects of suicide gene therapy in three HB cell lines using a yeast\u2010derived cytosine deaminase (YCD)\u2010combined yeast uracil phosphoribosyltransferase (YUPRT)\u2010based adenovirus\u2010mediated gene transfer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>YCD and YUPRT were fused to form the bifunctional suicide gene SuperCD. Adeonoviral vectors were used for transduction. Tumor cells transduced at MOI 50 were incubated with 5\u2010fluorocytosine (5\u2010FC) in ascending concentrations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Transduction rates were 87.8% (+6.7) in the mixed HB cell line HUH6, 98.6% (+1.4) in the epithelial HB cell line HepT1 and 93.6% (+0.6) in the multifocal HB embryonal cell line HepT3, respectively. In HepT3 and HepT1 cells suicide gene therapy with SuperCD\/5\u2010FC was highly effective leading to HB cell damage far above those of application of the prodrug 5\u2010FC only. In HUH6 cells the approach had no effect due to a lack in activity of the CMV promoter being employed for transcription of the SuperCD transgene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Assuming employment of fully active promoters, the SuperCD\/5\u2010FC approach may serve as a potentially useful anti\u2010tumor strategy against advanced HB. Pediatr Blood Cancer 2009;53:145\u2013151. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.21956","type":"journal-article","created":{"date-parts":[[2009,2,11]],"date-time":"2009-02-11T22:16:27Z","timestamp":1234390587000},"page":"145-151","source":"Crossref","is-referenced-by-count":11,"title":["Adenovirus\u2010mediated cytosine deaminase\/5\u2010fluorocytosine suicide gene therapy of human hepatoblastoma in vitro"],"prefix":"10.1002","volume":"53","author":[{"given":"Steven W.","family":"Warmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sorin","family":"Armeanu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heike","family":"Heigoldt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Ruck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Vonthein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heike","family":"Heitmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Seitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Luise","family":"Lemken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Bitzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Fuchs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich M.","family":"Lauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,11]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2003.06.003"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1998.9.17-2595"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1297-1354"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1089\/10430349950016979"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/10430340050016175"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.154.10.5302"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19970516)71:4<675::AID-IJC26>3.0.CO;2-9"},{"key":"e_1_2_5_9_2","first-page":"461","article-title":"In vivo gene therapy for alpha\u2010fetoprotein\u2010producing hepatocellular carcinoma by adenovirus\u2010mediated transfer of cytosine deaminase gene","volume":"57","author":"Kanai F","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_5_10_2","first-page":"773","volume":"62","author":"Miller CR","year":"2002","journal-title":"Intratumoral 5\u2010fluorouracil produced by cytosine deaminase\/5\u2010fluorocytosine gene therapy is effective for experimental human glioblastomas. 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