{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:57:29Z","timestamp":1791010649251,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,11,10]],"date-time":"2010-11-10T00:00:00Z","timestamp":1289347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Tyneside Leukaemia Research Association"},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The HLXB9 gene encodes a homeodomain containing transcription factor which has been implicated in the development of both solid and hematological malignancies. In leukemia it is one of the two fused genes, along with ETV6, in a recurrent translocation frequently observed in infant AML.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>Here we investigate the role of epigenetic inactivation of the HLXB9 gene in leukemia. Quantitative DNA methylation analysis was performed using the COBRA assay, and qRT\u2010PCR was used to assess the effects of methylation on expression in hematological cell lines and primary ALL samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hypermethylation of the HLXB9 gene was found to be a frequent event in childhood ALL, occurring in 33% of cases. However, it was rarely or never observed in other types of leukemia, including AML, CML, and CLL, with the exception of adult ALL, in which 39% of cases were hypermethylated. Furthermore, hypermethylation of HLXB9 results in loss of expression in hematological cell lines and primary ALL samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that HLXB9 may have a dual role in childhood leukemia, as an oncogene in infant AML but as a tumor suppressor in childhood ALL. Pediatr Blood Cancer 2011;56:349\u2013352. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.22679","type":"journal-article","created":{"date-parts":[[2010,11,10]],"date-time":"2010-11-10T17:08:27Z","timestamp":1289408907000},"page":"349-352","source":"Crossref","is-referenced-by-count":11,"title":["The dual role of HLXB9 in leukemia"],"prefix":"10.1002","volume":"56","author":[{"given":"Stuart","family":"Ferguson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah E.","family":"Gautrey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon","family":"Strathdee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,11,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/3828"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/302723"},{"key":"e_1_2_6_4_2","first-page":"5374","article-title":"Fusion of the homeobox gene HLXB9 and the ETV6 gene in infant acute myeloid leukemias with the t(7;12)(q36;p13)","volume":"61","author":"Beverloo HB","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/302899"},{"key":"e_1_2_6_6_2","first-page":"1479","article-title":"Expression and localization of homeodomain proteins DLX4\/HB9 in normal and malignant human breast tissues","volume":"23","author":"Neufing PJ","year":"2003","journal-title":"Anticancer Res"},{"key":"e_1_2_6_7_2","first-page":"955","article-title":"Expression and localization of homeodomain proteins DLX4, HB9 and HB24 in malignant and benign human colorectal tissues","volume":"24","author":"Hollington P","year":"2004","journal-title":"Anticancer Res"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20335"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20113"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra023075"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/35016125"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-6-45"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-2828"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0701059104"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.4161\/epi.9130"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-0919"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404254"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgl133"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.12.2532"},{"key":"e_1_2_6_20_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1182\/blood.V78.2.323.323","article-title":"Selective expression of two homeobox genes in CD34\u2010positive cells from human bone marrow","volume":"78","author":"Deguchi Y","year":"1991","journal-title":"Blood"}],"container-title":["Pediatric Blood &amp; Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpbc.22679","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpbc.22679","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pbc.22679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T13:42:58Z","timestamp":1696858978000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pbc.22679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,10]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["10.1002\/pbc.22679"],"URL":"https:\/\/doi.org\/10.1002\/pbc.22679","archive":["Portico"],"relation":{},"ISSN":["1545-5009","1545-5017"],"issn-type":[{"value":"1545-5009","type":"print"},{"value":"1545-5017","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11,10]]}}}