{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T09:57:01Z","timestamp":1760608621034},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,11,5]],"date-time":"2010-11-05T00:00:00Z","timestamp":1288915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Graft contamination has been blamed for causing relapse in children with high\u2010risk neuroblastoma (HRNB) after autologous hematopoietic stem cell transplantation (HSCT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>We report the long\u2010term results of hematopoietic reconstitution, post\u2010transplant complications, and clinical outcome of 44 children with HRNB treated with busulfan\/melphalan high\u2010dose chemotherapy followed by transplantation of purged CD34+ immunoselected autologous peripheral HSCT. Minimal residual disease (MRD) of grafts was evaluated by anti\u2010GD2 immunofluorescence or tyrosine hydroxylase reverse transcriptase\u2010polymerase chain reaction (RT\u2010PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Contaminating neuroblasts were found in 19\/38 grafts (50%) before CD34+ positive selection, and none after (technique sensitivity of one cell in 10<jats:sup>5<\/jats:sup>). A median of 6.5\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> CD34+ cells\/kg (range 0.8\u201323.7) were transplanted with only 2% of TRM. Neutrophils and platelet recovery occurred within a median of 12 days (range 9\u201347) and 44 days (range 12\u2013259), respectively, without any secondary graft failure. Twenty\u2010three percents of patients experienced a sepsis (10\/44) and 14% a pyelonephritis (6\/44). Recurrence of varicella zoster virus occurred in 21% of patients (9\/44). Negative RT\u2010PCR MRD within the leukapheresis product and <jats:italic>cis<\/jats:italic>\u2010retinoic acid therapy were significantly and independently associated to a better survival (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Overall and event\u2010free survivals at 5 years post\u2010transplant were at 59.3% and 48.3% respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Besides high rates of manageable infections due to late immune recovery, transplantation with CD34+ immunoselected grafts in HRNB children was feasible and did not affect long\u2010term hematopoiesis. Pediatr Blood Cancer. 2010;56:134\u2013142. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.22840","type":"journal-article","created":{"date-parts":[[2010,11,7]],"date-time":"2010-11-07T13:42:12Z","timestamp":1289137332000},"page":"134-142","source":"Crossref","is-referenced-by-count":14,"title":["CD34+ immunoselection of autologous grafts for the treatment of high\u2010risk neuroblastoma"],"prefix":"10.1002","volume":"56","author":[{"given":"Aur\u00e9lien","family":"Marabelle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Etienne","family":"Merlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascale","family":"Halle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Paillard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Berger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrei","family":"Tchirkov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rapha\u00ebl","family":"Rousseau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Leverger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Piguet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Louis","family":"Stephan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Demeocq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justyna","family":"Kanold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,11,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199910143411601"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"3085","DOI":"10.1182\/blood.V83.10.3085.3085","article-title":"Clonogenicity of circulating neuroblastoma cells: Implications regarding peripheral blood stem cell transplantation","volume":"83","author":"Moss TJ","year":"1994","journal-title":"Blood"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.2.380.380"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"3085","DOI":"10.1182\/blood.V83.10.3085.3085","article-title":"Clonogenicity of circulating neuroblastoma cells: Implications regarding peripheral blood stem cell transplantation","volume":"83","author":"Moss T","year":"1994","journal-title":"Blood"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19910715)68:2<242::AID-CNCR2820680204>3.0.CO;2-P"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(97)00227-X"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)90004-7"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1986.239"},{"key":"e_1_2_6_10_2","first-page":"5882","article-title":"Model system for removing neuroblastoma cells from bone marrow using monoclonal antibodies and magnetic immunobeads","volume":"46","author":"Reynolds CP","year":"1986","journal-title":"Cancer Res"},{"key":"e_1_2_6_11_2","first-page":"5001","article-title":"Efficacy and toxicity of a virus\u2010directed enzyme prodrug therapy purging method: Preclinical assessment and application to bone marrow samples from neuroblastoma patients","volume":"62","author":"Wagner LM","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1996.14.8.2224"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1995.tb05318.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1700876"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-911X(199804)30:4<228::AID-MPO4>3.0.CO;2-I"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-9987.1998.tb00073.x"},{"key":"e_1_2_6_17_2","first-page":"1004","article-title":"The pros and cons of split\u2010dose granulocyte colony\u2010stimulating factor alone rather than a single high dose for hematopoietic progenitor cell mobilization in small children (<15kg) with solid tumors","volume":"91","author":"Merlin E","year":"2006","journal-title":"Haematologica"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-911X(199901)32:1<7::AID-MPO3>3.0.CO;2-Z"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/106161299320488"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.1.1996.5.213"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/152581600419206"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00442474"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1986.220"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1986.4.3.363"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1089\/152581603322286060"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1701737"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.1.1997.6.235"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703536"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1991.9.6.1050"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(95)00015-B"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3046.1999.00070.x"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1987.5.2.266"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1053\/bbmt.1998.v4.pm9701390"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2007.01.074"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.1.1998.7.367"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2000.7930145.x"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.2.754"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704402"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-126-8-199704150-00002"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-126-8-199704150-00002"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(97)00243-8"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1701471"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V83.3.636.636"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11389"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/1096-911X(20001201)35:6<677::AID-MPO42>3.0.CO;2-H"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0740"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/1096-911X(20010101)36:1<213::AID-MPO1052>3.0.CO;2-9"},{"key":"e_1_2_6_48_2","first-page":"1021","article-title":"Prognostic impact of the detection of microcirculating tumor cells by a real\u2010time RT\u2010PCR assay of tyrosine hydroxylase in patients with advanced neuroblastoma","volume":"14","author":"Parareda A","year":"2005","journal-title":"Oncol Rep"},{"key":"e_1_2_6_49_2","first-page":"121","article-title":"Comparison of immunocytochemistry real\u2010time quantitative RT\u2010PCR and flow cytometry for detection of minimal residual disease in neuroblastoma","volume":"27","author":"Ifversen MR","year":"2005","journal-title":"Int J Oncol"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6601014"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-05-155457"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20091921"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20091918"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703536"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(05)70291-6"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.20219"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(97)00324-9"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2000.18.3.468"},{"key":"#cr-split#-e_1_2_6_59_2.1","doi-asserted-by":"crossref","unstructured":"KreissmanS. A randomized phase III trial of myeloablative autologous peripheral blood stem cell (PBSC) transplant (ASCT) for high\u2010risk neuroblastoma (HR\u2010NB) employing immunomagnetic purged (P) versus unpurged (UP) PBSC: A Children's Oncology Group study. J Clin Oncol 26: 2008 (May 20 suppl","DOI":"10.1200\/jco.2008.26.15_suppl.10011"},{"key":"#cr-split#-e_1_2_6_59_2.2","unstructured":"abstr 10011) 2008:http:\/\/www.asco.org\/ASCOv2\/Meetings\/Abstracts?&vmview=abst_detail_view&confID=55&abstractID=30386."},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.13.8925"}],"container-title":["Pediatric Blood &amp; Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpbc.22840","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpbc.22840","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pbc.22840","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T08:34:38Z","timestamp":1696926878000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pbc.22840"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,5]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["10.1002\/pbc.22840"],"URL":"https:\/\/doi.org\/10.1002\/pbc.22840","archive":["Portico"],"relation":{},"ISSN":["1545-5009","1545-5017"],"issn-type":[{"value":"1545-5009","type":"print"},{"value":"1545-5017","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11,5]]}}}