{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:10:16Z","timestamp":1761919816512},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,3,26]],"date-time":"2014-03-26T00:00:00Z","timestamp":1395792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Burkitt lymphoma\/leukemia (BL\/L) is cytogenetically characterized by the t(8;14)(q24;q32) or its variants, t(2;8)(p11;q21), and t(8;22)(q24;q11.2), which juxtapose the <jats:italic>MYC<\/jats:italic> oncogene to one of the three immunoglobulin loci. The overall cure rate of BL\/L in children is 70\u201390%, but patients diagnosed with advanced\u2010stage disease have a less favorable prognosis. It is possible that secondary chromosomal abnormalities contribute to this unfavorable prognosis via chemotherapy resistance, but the results of genetic studies have been inconsistent. This study aimed to identify and characterize secondary chromosomal abnormalities associated with the t(8;14) and its variants in children with French\u2010American\u2010British\u2010L3 leukemia or Burkitt lymphoma with bone marrow involvement at the time of diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>Chromosome analysis was based on G\u2010banding. Fluorescence <jats:italic>in situ<\/jats:italic> hybridization technique was applied using <jats:italic>IGH\/MYC\/CEP8<\/jats:italic> dual\u2010fusion and <jats:italic>MYC<\/jats:italic> break\u2010apart probes. Multicolor chromosome banding was performed according to standard protocol.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We describe a group of BL\/L with extreme adverse clinical outcome, in which secondary chromosomal abnormalities, particularly those involving the long arms of chromosomes 1 and 13, were found in 71% of cases. The <jats:italic>IGH\/MYC<\/jats:italic> fusion showed molecular heterogeneity in 14% of cases and two cases exhibited three <jats:italic>IGH\/MYC<\/jats:italic> fusion signals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Secondary chromosomal abnormalities were found in a high proportion of patients. We observed an extent of <jats:italic>IGH\/MYC<\/jats:italic> heterogeneity not previously reported in Burkitt lymphoma, including the novel finding of three fusion signals in two cases. Pediatr Blood Cancer 2014; 61:1422\u20131426. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.25030","type":"journal-article","created":{"date-parts":[[2014,3,26]],"date-time":"2014-03-26T10:29:10Z","timestamp":1395829750000},"page":"1422-1426","source":"Crossref","is-referenced-by-count":7,"title":["Conventional and molecular cytogenetic characterization of Burkitt lymphoma with bone marrow involvement in Brazilian children and adolescents"],"prefix":"10.1002","volume":"61","author":[{"given":"Mariana T.","family":"De Souza","sequence":"first","affiliation":[{"name":"Post Graduation Program in Oncology National Cancer Institute Rio de Janeiro RJ Brazil"},{"name":"Cytogenetics Department, Bone Marrow Transplantation Unit National Cancer Institute Rio de Janeiro RJ Brazil"},{"name":"National Institute of Science and Technology for Cancer Control Rio de Janeiro 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