{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T17:09:26Z","timestamp":1793898566620,"version":"4.8.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T00:00:00Z","timestamp":1557187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"MH CZ-DRO, Motol University Hospital, Prague, Czech Republic"},{"name":"Floga-Parents Association of Children with Cancer"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Bone marrow (BM) samples obtained from minimal residual disease (MRD)\u2010negative children with B\u2010cell acute lymphoblastic leukemia (B\u2010ALL) were used in our laboratory as negative biological controls for the development of a neuroblastoma (NBL) flow\u2010cytometric (FC) protocol. The accidental, but systematic, identification of rare cell populations (RCP) mimicking NBL cells (CD45<jats:sup>\u2212<\/jats:sup>\/CD56<jats:sup>+<\/jats:sup>) in these samples indicated the need for their thorough immunophenotypic identification, in order to elucidate their possible interference in NBL\u2010MRD assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>RCP observed in BM samples from 14 children recovering from BM aplasia due to intensive chemotherapy for B\u2010ALL were investigated with the following markers: CD81, CD200, CD24, GD2, CD73, CD13, CD90, CD146, CD9, CD117, CD10, CD99, and NG2. BM samples from six newly diagnosed patients with NBL and an NBL cell line were simultaneously investigated as positive controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The frequency of RCP in B\u2010ALL BM samples was\u00a0&lt;\u00a01\/1\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup> cells (bulky lysis), and their immunophenotypic profile was indicative of CD56<jats:sup>+<\/jats:sup> mesenchymal stromal cells (MSCs) (CD45<jats:sup>\u2212<\/jats:sup>, CD90<jats:sup>+<\/jats:sup>, CD146<jats:sup>+<\/jats:sup>, CD73<jats:sup>+<\/jats:sup>). Also, RCP expressed CD81 and CD200, simulating NBL cells. The most useful discriminative markers for CD56<jats:sup>+<\/jats:sup> MSCs were CD13 and CD73. An appropriate protocol consisting of two tubes with seven color combinations was further proposed: SYTO\u201016, GD2 (first tube) or CD73 (second tube)\u2010PE, CD24\u2010ECD, CD13\u2010PC5.5, CD45\u2010PC7, CD81\u2010APC, and CD56\u2010APC700.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>RCP that were immunophenotypically similar to NBL were identified as CD56<jats:sup>+<\/jats:sup> MSCs. As these cells might pose an obstacle to accurate NBL disease assessment by FC, especially MRD, an enhanced NBL\u2010FC protocol is proposed for prospective evaluation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.27799","type":"journal-article","created":{"date-parts":[[2019,5,8]],"date-time":"2019-05-08T06:16:21Z","timestamp":1557296181000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Interference of bone marrow CD56<sup>+<\/sup> mesenchymal stromal cells in minimal residual disease investigation of neuroblastoma and other CD45<sup>\u2212<\/sup>\/CD56<sup>+<\/sup> pediatric malignancies using flow cytometry"],"prefix":"10.1002","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1794-6332","authenticated-orcid":false,"given":"Ioannis","family":"Theodorakos","sequence":"first","affiliation":[{"name":"Flow Cytometry Laboratory, Department of Immunology Athens Regional General Hospital \u201cG. Gennimatas,\u201d  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios","family":"Paterakis","sequence":"additional","affiliation":[{"name":"Flow Cytometry Laboratory, Department of Immunology Athens Regional General Hospital \u201cG. Gennimatas,\u201d  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vassilios","family":"Papadakis","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology\u2010Oncology \u201cAghia Sophia\u201d Children's Hospital  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ales","family":"Vicha","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology Charles University, 2nd Faculty of Medicine and Faculty Hospital Motol, Prague  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios","family":"Topakas","sequence":"additional","affiliation":[{"name":"Flow Cytometry Laboratory, Department of Immunology Athens Regional General Hospital \u201cG. Gennimatas,\u201d  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavla","family":"Jencova","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology Charles University, 2nd Faculty of Medicine and Faculty Hospital Motol, Prague  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eirini","family":"Karchilaki","sequence":"additional","affiliation":[{"name":"Flow Cytometry Laboratory, Department of Immunology Athens Regional General Hospital \u201cG. Gennimatas,\u201d  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Taparkou","sequence":"additional","affiliation":[{"name":"1st Department of Pediatrics Aristotle University of Thessaloniki, Hippokration Hospital  Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaos J.","family":"Tsagarakis","sequence":"additional","affiliation":[{"name":"Flow Cytometry Laboratory, Department of Immunology Athens Regional General Hospital \u201cG. 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