{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T23:02:58Z","timestamp":1789599778275,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T00:00:00Z","timestamp":1573689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Use of serum hepcidin measurements in pediatrics would benefit from standardized age\u2010 and sex\u2010specific reference ranges in children, in order to enable the establishment of clinical decision limits that are universally applicable.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>We measured serum hepcidin\u201025 levels in 266 healthy Dutch children aged 0.3\u201017 years, using an isotope dilution mass spectrometry assay, standardized with our commutable secondary reference material (RM), assigned by a candidate primary RM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We constructed age\u2010 and sex\u2010specific values for serum hepcidin and its ratio with ferritin and transferrin saturation (TSAT). Serum hepcidin levels and hepcidin\/ferritin and TSAT\/hepcidin ratios were similar for both sexes. Serum hepcidin and hepcidin\/ferritin ratio substantially declined after the age of 12 years and TSAT\/hepcidin ratio gradually increased with increasing age. Serum hepcidin values for Dutch children &lt;12 years (n\u00a0=\u00a0170) and &gt;12 years (n\u00a0=\u00a096) were 1.9\u00a0nmol\/L (median); 0.1\u201013.1\u00a0nmol\/L (p2.5\u2010p97.5) and 0.9\u00a0nmol\/L; 0.0\u20109.1\u00a0nmol\/L, respectively. Serum ferritin was the most significant correlate of serum hepcidin in our study population, explaining 15.1% and 7.9% of variance in males and females, respectively. Multivariable linear regression analysis including age, blood sampling time, iron parameters, ALT, CRP, and body mass index as independent variables showed a statistically significant negative association between age as a dichotomous variable (\u226412 vs &gt;12 years) and log\u2010transformed serum hepcidin levels in both sexes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We demonstrate that serum hepcidin relative to indicators of body iron is age dependent in children, suggesting that the set point of serum hepcidin relative to stored and circulating iron changes during childhood.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.28038","type":"journal-article","created":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T11:20:38Z","timestamp":1573730438000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Standardized serum hepcidin values in Dutch children: Set point relative to body iron changes during childhood"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9852-515X","authenticated-orcid":false,"given":"Albertine E.","family":"Donker","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Radboud Institute for Molecular Life Sciences Radboud University Medical Center  Nijmegen The Netherlands"},{"name":"Department of Pediatrics M\u00e1xima Medical Center  Veldhoven The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tessel E.","family":"Galesloot","sequence":"additional","affiliation":[{"name":"Radboud Institute for Health Sciences Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coby M.","family":"Laarakkers","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Radboud Institute for Molecular Life Sciences Radboud University Medical Center  Nijmegen The 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