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An increase in the classical monocyte fraction (CD14++\/CD16\u2212) greater than 94.0% of total monocytes is considered highly sensitive and specific in distinguishing chronic myelomonocytic leukemia from other myeloproliferative disorders. In a pilot study of juvenile myelomonocytic leukemia cases, we noted that CD14++\/CD16\u2212 monocyte fraction was\u00a0&gt;95% in de novo juvenile myelomonocytic leukemia (JMML) with somatic <jats:italic>PTPN11<\/jats:italic> mutations but normal in those with monosomy 7 or Noonan syndrome. Monocyte subgroup profiling by itself is not diagnostic of JMML but may distinguish molecular subgroups within JMML.<\/jats:p>","DOI":"10.1002\/pbc.28555","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T12:42:56Z","timestamp":1594384976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["CD14\/16 monocyte profiling in juvenile myelomonocytic leukemia"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8366-9487","authenticated-orcid":false,"given":"Manisha","family":"Gadgeel","sequence":"first","affiliation":[{"name":"School of Medicine, Wayne State University  Detroit Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4978-0346","authenticated-orcid":false,"given":"Shruti","family":"Bagla","sequence":"additional","affiliation":[{"name":"School of Medicine, Wayne State University  Detroit Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Buck","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology Children's Hospital of Michigan  Detroit Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Shamoun","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology Children's Hospital of Michigan  Detroit Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaddanapudi","family":"Ravindranath","sequence":"additional","affiliation":[{"name":"School of Medicine, Wayne State University  Detroit Michigan"},{"name":"Division of Hematology\/Oncology Children's Hospital of Michigan  Detroit Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1309\/HRJ1XKTD77J1UTFM"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-12-753210"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-01-620781"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2018.00109"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V74.7.2527.2527"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-02-258558"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200101)31:1<48::AID-IMMU48>3.0.CO;2-5"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/bcj.2017.66"},{"key":"e_1_2_7_10_1","article-title":"Pattern of human monocyte subpopulations in health and disease","author":"Ozanska A","year":"2020","journal-title":"Scand J Immunol"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/imcb.12337"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V69.3.975.975"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197705)39:5<2017::AID-CNCR2820390518>3.0.CO;2-Z"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-09-667808"},{"issue":"5","key":"e_1_2_7_15_1","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.MCHD-0015-2015","article-title":"Monocyte, macrophage, and dendritic cell development: the human perspective","volume":"4","author":"Collin M","year":"2016","journal-title":"Microbiol Spectr"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.10.005"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07801-x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3400"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-11-844688"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-07-798157"}],"container-title":["Pediatric Blood &amp; 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