{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T07:38:13Z","timestamp":1776757093598,"version":"3.51.2"},"reference-count":19,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cytoreductive surgery in combination with hyperthermic intraperitoneal chemotherapy has been established as a novel treatment approach for peritoneal sarcomatosis. Despite promising clinical reports, there is still a lack of knowledge regarding optimal drug usage and local effects. Therefore, we intended to establish a murine animal model for further evaluation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>Alveolar rhabdomyosarcoma cells were xenotransplanted into NOD\/LtSz\u2010scid IL2R\u03b3nullmice (<jats:italic>n<\/jats:italic>\u00a0=\u00a0100). The mice received a continuous intraperitoneal lavage with isotonic saline solution as control or with cisplatin (30 or 60\u00a0mg\/m<jats:sup>2<\/jats:sup>) as treatment group for 60\u00a0minutes at 37\u00b0C\u00a0or 42\u00b0C (6\u00a0subgroups, each <jats:italic>n<\/jats:italic>\u00a0=\u00a016). Tumor spread was documented by an adapted peritoneal cancer index and MRI (<jats:italic>n<\/jats:italic>\u00a0=\u00a04). Tumor and tissue samples, harvested at the end of the perfusion, were evaluated regarding morphology, proliferation, and apoptosis (H&amp;E\u2010, Ki\u201067\u2010, cleaved caspase 3\u2013staining, TUNEL assay).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Extensive peritoneal sarcomatosis in over 91% of the cases was observed. HIPEC was feasible without acute side effects. Ki\u201067 staining revealed concentration\u2010 or temperature\u2010dependent effects of cisplatin\u2010based HIPEC on the tumors. Although cleaved caspase\u20103 showed only sporadic apoptotic effects. TUNEL assay detected concentration\u2010 or temperature\u2010dependent apoptotic effects at the outer tumor surface. MRI scans confirmed the observed tumor dissemination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first animal model for evaluation of HIPEC in pediatric RMS in mice. Cisplatin\u2010based HIPEC had early effects on the proliferation whereas circumscribed apoptotic effects could be detected at the tumor surface. This model allows further insights on the possible efficiency of HIPEC in RMS. Further studies using other drug combinations and treatment will follow.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.29202","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T04:22:01Z","timestamp":1625199721000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Establishment of a new valid animal model for the evaluation of hyperthermic intraperitoneal chemotherapy (HIPEC) in pediatric rhabdomyosarcoma"],"prefix":"10.1002","volume":"68","author":[{"given":"Benedikt R.","family":"Wagner","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery University Hospital Marburg  Marburg Baldingerstrasse 35033 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna L.","family":"Adamus","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery University Hospital Marburg  Marburg Baldingerstrasse 35033 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00f6rthe","family":"S\u00f6nnecken","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery University Hospital Marburg  Marburg Baldingerstrasse 35033 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Vahdad","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery University Hospital Marburg  Marburg Baldingerstrasse 35033 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Jank","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospital Marburg  Marburg Baldingerstrasse 35033 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carsten","family":"Denkert","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospital Marburg  Marburg Baldingerstrasse 35033 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Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24293"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199501000-00004"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-012-2229-2"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-014-4289-y"},{"key":"e_1_2_10_6_1","doi-asserted-by":"crossref","unstructured":"GescheJ BeckertS NeunhoefferF et\u00a0al.Cytoreductive surgery and hyperthermic intraperitoneal chemotherapy: a safe treatment option for intraperitoneal rhabdomyosarcoma in children below 5 years of age.Pediatr Blood Cancer.2019;66(2):e27517. 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