{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:17:45Z","timestamp":1792808265578,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:00:00Z","timestamp":1657584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cytoreductive surgery (CRS) in combination with hyperthermic intraperitoneal chemotherapy (HIPEC) is an option in advanced peritoneal sarcomatosis. Nevertheless, CRS and HIPEC are not successful in all patients. An enhancement of HIPEC using photodynamic therapy (PDT) might be beneficial. Therefore, a combination of the photosensitizer hypericin (HYP) with HIPEC was evaluated in an animal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>An established HIPEC animal model for rhabdomyosarcoma (NOD\/LtSz\u2010scid IL2R\u03b3nullmice, <jats:italic>n<\/jats:italic>\u00a0=\u00a080) was used. All groups received HYP (100\u00a0\u03bcg\/200\u00a0\u03bcl) intraperitoneally with and without cisplatin\u2010based (30 or 60\u00a0mg\/m<jats:sup>2<\/jats:sup>) HIPEC (37\u00b0C or 42\u00b0C, for 60\u00a0minutes) (five groups, each <jats:italic>n<\/jats:italic>\u00a0=\u00a016). Peritoneal cancer index (PCI) was documented visually and by HYP\u2010based photodynamic diagnosis (PDD). HYP\u2010based PDT of the tumor was performed. Tissue samples were evaluated regarding proliferation (Ki\u201067) and apoptosis (TUNEL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HYP uptake was detected even in smallest tumor nodes (&lt;1\u00a0mm) with improved tumor detection during PDD (PCI with PDD vs. PCI without PDD: 8.5 vs. 7, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001***). Apoptotic effects after PDT without HIPEC were limited to the tumor surface, whereas PDT after HIPEC (60\u00a0mg\/m<jats:sup>2<\/jats:sup>, 42\u00b0C) showed additional reduction of tumor proliferation in the top nine to 11 cell layers (50\u00a0\u03bcm).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HYP as fluorescent photosensitizer offers an intraoperative diagnostic advantage detecting intraperitoneal tumor dissemination. The combination of HYP and cisplatin\u2010based HIPEC was feasible in vivo, showing enhanced effects on tumor proliferation and apoptosis induction across the tumor surface. Further studies combining HYP and HIPEC will follow to establish a clinical application.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.29864","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T15:46:52Z","timestamp":1655912812000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Increasing the efficiency of hyperthermic intraperitoneal chemotherapy (HIPEC) by combination with a photosensitive drug in pediatric rhabdomyosarcoma in an animal model"],"prefix":"10.1002","volume":"69","author":[{"given":"Benedikt R.","family":"Wagner","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery University Hospital Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna L.","family":"Adamus","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery University Hospital Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Hempfling","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery University Hospital Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Vahdad","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery University Hospital Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antje","family":"Haap\u2010Hoff","sequence":"additional","affiliation":[{"name":"KARL STORZ SE &amp; Co. 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