{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:38:28Z","timestamp":1785883108936,"version":"3.56.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005762","name":"National Taiwan University Hospital","doi-asserted-by":"publisher","award":["108\u2010S4255"],"award-info":[{"award-number":["108\u2010S4255"]}],"id":[{"id":"10.13039\/501100005762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Acute lymphoblastic leukemia (ALL) is the most common pediatric cancer. Minimal residual disease (MRD) detection is the most powerful prognostic tool for monitoring treatment efficacy and predicting clinical outcomes. We aimed to identify key leukemia\u2010associated markers, the proportions of differential expression in patients, and the most effective marker combination for MRD detection by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bone marrow samples were collected from 132 pediatric patients with newly diagnosed (<jats:italic>n<\/jats:italic>\u00a0=\u00a0115) or relapsed (<jats:italic>n<\/jats:italic>\u00a0=\u00a017) B\u2010cell precursor (BCP)\u2010ALL. We used CD19, CD10, CD34, CD45 as backbone markers to identify immature B cells and analyzed the differential expression of 18 leukemia\u2010associated markers using seven\u2010color multiparameter flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Leukemic cells in all 132 patients expressed leukemia\u2010associated markers. The most commonly overexpressed marker was heat shock protein 27 (Hsp27) (108 patients, 81%), followed by CD73 (102 patients, 77%) and CD123 (80 patients, 60%). CD38 was underexpressed in 64 patients (48%). Hsp27 overexpression persisted in 50 out of 57 follow\u2010up MRD bone marrow samples (87%) and was associated with older age at diagnosis. Hsp27 overexpression was not associated with MRD levels or genetic abnormalities including hyperdiploidy, t(12;21)<jats:italic>\/ETV6\u2010RUNX1<\/jats:italic>, t(1;19)\/<jats:italic>TCF3\u2010PBX1<\/jats:italic>, t(9;22)\/<jats:italic>BCR\u2010ABL1<\/jats:italic>, or 11q23\/<jats:italic>KMT2A<\/jats:italic> rearrangements. Four remaining leukemia\u2010associated markers (Hsp27, CD73, CD58, CD24) after <jats:italic>in silico<\/jats:italic> deletion from the original panel could collectively detect leukemia\u2010associated cell profiles in 100% of cases in this cohort and 98% of cases in a validation cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hsp27 combined with CD73, CD58, CD24, and backbone markers allows monitoring MRD in virtually all patients with BCP\u2010ALL.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.29990","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T15:11:37Z","timestamp":1666019497000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["High frequency of heat shock protein 27 overexpression is a highly effective, high\u2010coverage marker for minimal residual disease detection in children with B\u2010cell acute lymphoblastic leukemia"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4562-6002","authenticated-orcid":false,"given":"Shu\u2010Wei","family":"Chou","sequence":"first","affiliation":[{"name":"Department of Pediatrics National Taiwan University Hospital, National Taiwan University College of Medicine  Taipei Taiwan"},{"name":"Graduate Institute of Clinical Medicine National Taiwan University College of Medicine  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying\u2010Hui","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Pediatrics National Taiwan University Hospital, National Taiwan University College of Medicine  Taipei Taiwan"},{"name":"Department of Laboratory Medicine National Taiwan University Hospital, National Taiwan University College of Medicine  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng\u2010Yao","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics National Taiwan University Hospital, National Taiwan University College of Medicine  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiu\u2010Hao","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics National Taiwan University Hospital, National Taiwan University College of Medicine  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3598-7218","authenticated-orcid":false,"given":"Yung\u2010Li","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics National Taiwan University Hospital, National Taiwan University College of Medicine  Taipei Taiwan"},{"name":"Department of Laboratory Medicine National Taiwan University Hospital, National Taiwan University College of Medicine  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010Tsamn","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics National Taiwan University Hospital, National Taiwan University College of Medicine  Taipei Taiwan"},{"name":"Department of Laboratory Medicine National Taiwan University Hospital, National Taiwan University College of Medicine  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai\u2010Hsin","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics National Taiwan University Hospital, National Taiwan University College of Medicine  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine","family":"Coustan\u2010Smith","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Yong Loo Lin School of Medicine National University of Singapore  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