{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:55:49Z","timestamp":1790700949704,"version":"4.1.0"},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T00:00:00Z","timestamp":1668470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970111"],"award-info":[{"award-number":["81970111"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Immune thrombocytopenia (ITP) is an autoimmune\u2010mediated hemorrhagic disease. Anti\u2010glycoprotein autoantibodies play a key role in the pathophysiology of ITP, but the relationship between platelet\u2010specific antibodies and bleeding severity is unclear. This study aimed to analyze the relationship between anti\u2010glycoprotein autoantibodies and bleeding severity in children with newly diagnosed ITP and platelet count less than 10 \u00d7 10<jats:sup>9<\/jats:sup>\/L.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>This was a single\u2010center prospective observational study that analyzed children with newly diagnosed ITP and platelet count less than 10 \u00d7 10<jats:sup>9<\/jats:sup>\/L between June 2018 and September 2021 at our hospital. The children were classified into the mild and severe groups based on the bleeding scores. The type and titer of anti\u2010glycoprotein autoantibodies were detected using an enzyme\u2010linked immunosorbent assay (ELISA) kit (PAKAUTO). We analyzed the relationship between bleeding severity and anti\u2010glycoprotein autoantibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 86 cases were enrolled, including 42 in the mild group and 44 in the severe group. Patients with anti\u2010GPIIb\/IIIa or anti\u2010GPIb\/IX antibodies suffered more severe bleeding than patients without them (<jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> = 7.303, <jats:italic>p<\/jats:italic> = .007; <jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> = 3.875, <jats:italic>p<\/jats:italic> = .049), but there was no significant difference between patients with or without anti\u2010GPIa\/IIa antibodies (<jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> = 0.745, <jats:italic>p<\/jats:italic> = .388). When antibodies were analyzed together, patients with three antibodies suffered more severe bleeding than those without three antibodies (<jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> = 5.053, <jats:italic>p<\/jats:italic> = .025). Patients with higher antibody titer in the eluent, but not in the plasma, suffered more severe bleeding in all three antibodies (<jats:italic>Z<\/jats:italic> = \u22122.389, <jats:italic>p<\/jats:italic> = .017; <jats:italic>Z<\/jats:italic> = \u22122.108, <jats:italic>p<\/jats:italic> = .035; <jats:italic>Z<\/jats:italic> = \u22122.557, <jats:italic>p<\/jats:italic> = .011).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Anti\u2010glycoprotein autoantibodies led to more severe bleeding in children under 18\u00a0years of age without drug treatment with newly diagnosed ITP and platelet count less than 10\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/L.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.30094","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T11:34:05Z","timestamp":1668512045000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Anti\u2010glycoprotein autoantibodies are related to bleeding severity in children with newly diagnosed ITP and very low platelet counts"],"prefix":"10.1002","volume":"70","author":[{"given":"Shuyue","family":"Dong","sequence":"first","affiliation":[{"name":"Hematology Center Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital Capital Medical University National Center for Children's Health  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4278-8559","authenticated-orcid":false,"given":"Hao","family":"Gu","sequence":"additional","affiliation":[{"name":"Hematologic Disease Laboratory Beijing Pediatric Research Institute Beijing Children's Hospital, Capital Medical University  Beijing China"},{"name":"Department of Immunology Ministry of Education Key Laboratory of Major Diseases in Children Beijing Children's Hospital Capital Medical University National Center for Children's Health  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hematology Center Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital Capital Medical University National Center for Children's Health  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Fu","sequence":"additional","affiliation":[{"name":"Hematology Center Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital Capital Medical University National Center for Children's Health  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingjuan","family":"Xie","sequence":"additional","affiliation":[{"name":"Hematology Center Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital Capital Medical University National Center for Children's Health  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyao","family":"Ma","sequence":"additional","affiliation":[{"name":"Hematology Center Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital Capital Medical University National Center for Children's Health  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6759-075X","authenticated-orcid":false,"given":"Jie","family":"Ma","sequence":"additional","affiliation":[{"name":"Hematology Center Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital Capital Medical University National Center for Children's Health  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5273-145X","authenticated-orcid":false,"given":"Zhenping","family":"Chen","sequence":"additional","affiliation":[{"name":"Hematologic Disease Laboratory Beijing Pediatric Research Institute Beijing Children's Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4030-209X","authenticated-orcid":false,"given":"Runhui","family":"Wu","sequence":"additional","affiliation":[{"name":"Hematology Center Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital Capital Medical University National Center for Children's Health  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1683416"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1913324"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2013.03.001"},{"issue":"10","key":"e_1_2_9_5_1","first-page":"810","article-title":"[Adapted guideline for the diagnosis and treatment of primary immune thrombocytopenia for Chinese children (2021)]","volume":"59","author":"Working Group of Chinese Guideline for the Diagnosis and Treatment of Childhood Primary Immune Thrombocytopenia","year":"2021","journal-title":"Zhonghua Er Ke Za 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