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The aim of our study was to compare the prognostic power of repeated MFC\u2013MRD measurement with single\u2010point MRD assessment in children with BCP\u2010ALL treated with the reduced\u2010intensity protocol ALL\u2010MB 2008. Data from consecutive MFC\u2013MRD at day 15 and day 36 (end of induction, EOI) were available for 507 children with Philadelphia\u2010negative BCP\u2010ALL. They were stratified into standard risk (SR, <jats:italic>n<\/jats:italic>\u00a0=\u00a0265), intermediate risk (ImR, <jats:italic>n<\/jats:italic>\u00a0=\u00a0211), and high risk (HR, <jats:italic>n<\/jats:italic>\u00a0=\u00a031) according to the initial clinical characteristics defined in the ALL\u2010MB 2008 protocol. Quantitative (relative to quantitative thresholds) and kinetic (logarithmic reduction) assessments of MFC\u2013MRD at both time points effectively separated patients into three groups with different risk of recurrence. On the other hand, starting with low (for the SR group) and moderate (for the ImR group) induction therapy, a single MFC\u2013MRD measurement at EOI proved sufficient to unequivocally identify patients in whom this therapy is highly effective and distinguish them from those who cannot be successfully treated with such therapy. Therefore, initiating treatment with low or moderate treatment from the start, together with careful consideration of initial clinical risk factors and just one EOI\u2013MFC\u2013MRD measurement is simple, inexpensive, and entirely sufficient for treatment optimization. Furthermore, for a large proportion of patients, this approach allows better adjustment, in particular also reduction of therapy intensity than sequential MRD measurements.<\/jats:p>","DOI":"10.1002\/pbc.30295","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T14:29:39Z","timestamp":1680013779000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A simple procedure to identify children with B\u2010lineage acute lymphoblastic leukemia who can be successfully treated with low or moderate intensity: Sequential versus single\u2010point minimal residual disease measurement"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0889-6986","authenticated-orcid":false,"given":"Alexander","family":"Popov","sequence":"first","affiliation":[{"name":"National Research and Clinical Center for Pediatric Hematology Oncology and Immunology  Moscow Russian 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