{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:03:25Z","timestamp":1791644605547,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,7]],"date-time":"2023-05-07T00:00:00Z","timestamp":1683417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Experimental fertility preservation programs have been started to safeguard the future fertility of prepubertal and pubertal males requiring high\u2010risk gonadotoxic treatment protocols. However, long\u2010term follow\u2010up studies evaluating the effects on their gonadal development and function related to the testicular biopsy procedure are rather limited.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>This two\u2010center follow\u2010up study (between 2002 and 2020) evaluated the gonadal development and function of a cohort of 59 prepubertal and pubertal males who have been offered immature testicular tissue banking (TTB) prior to conventional high\u2010risk chemo\u2010 and\/or radiotherapy (HR\u2010C\/R) or conditioning therapy before hematopoietic stem cell transplantation (CT\u2010HSCT). The aim is to investigate the long\u2010term impact of the testicular biopsy procedure and the high\u2010risk gonadotoxic treatment. Testicular growth and the reproductive hormones luteinizing hormone (LH), follicle\u2010stimulating hormone (FSH), testosterone (T), and inhibin B (INHB) were analyzed after treatment completion, and compared between males accepting TTB and those refusing TTB (control) as well as between HR\u2010C\/R and CT\u2010HSCT treatment protocols.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 59 prepubertal and pubertal males included, 25 were treated by HR\u2010C\/R and 34 required CT\u2010HSCT. TTB was accepted for 39 males and refused for 20 males. Most patients were prepubertal at diagnosis (85%), at TTB (79%), and at treatment completion (76%), and pubertal or postpubertal at their last follow\u2010up visit (66%). After 5.0 (1.0\u201313.0) years post treatment, most patients show normal testicular volumes (83%) and normal LH (89%), FSH (87%), T (87%), and INHB (79%) serum levels. The testicular biopsy procedure did not have an effect on testicular growth, LH, FSH, T, and INHB. Significantly more small postpubertal testicular volumes (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0278) and low INHB serum levels (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0130) were recorded after CT\u2010HSCT, especially after myeloablative conditioning.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The clinical follow\u2010up data demonstrate no effect related to the biopsy procedure, but a substantial risk for impaired gonadal development after high\u2010risk gonadotoxic treatment, in particular myeloablative CT\u2010HSCT. Longer follow\u2010up studies with a larger study population are needed to confirm these preliminary findings.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.30370","type":"journal-article","created":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T05:14:57Z","timestamp":1683522897000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Gonadal development and function after immature testicular tissue banking as part of high\u2010risk gonadotoxic treatment"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3483-6511","authenticated-orcid":false,"given":"Aude","family":"Braye","sequence":"first","affiliation":[{"name":"Department of Reproduction, Genetics and Regenerative Medicine (RGRG), Biology of the Testis (BITE) Vrije Universiteit Brussel (VUB), Laarbeeklaan  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Delgouffe","sequence":"additional","affiliation":[{"name":"Department of Reproduction, Genetics and Regenerative Medicine (RGRG), Biology of the Testis (BITE) Vrije Universiteit Brussel (VUB), Laarbeeklaan  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jutte","family":"van der Werff ten Bosch","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Division of Pediatric Oncology Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Laarbeeklaan  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge","family":"Gies","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Division of Pediatric Endocrinology Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Laarbeeklaan  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Ferster","sequence":"additional","affiliation":[{"name":"Department of Hemato\u2010Oncology Universit\u00e9 Libre de Bruxelles (ULB), H\u00f4pital Universitaire des Enfants Reine Fabiola (HUDERF), Jean Joseph Crocqlaan  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Goossens","sequence":"additional","affiliation":[{"name":"Department of Reproduction, Genetics and Regenerative Medicine (RGRG), Biology of the Testis (BITE) Vrije Universiteit Brussel (VUB), Laarbeeklaan  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/0284186X.2015.1008648"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201018\u20100221"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213\u20108587(15)00038\u20108"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.21261"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/hsw\/hlx047"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.10.8993"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00520\u2010010\u20100958\u2010y"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/1179558119886342"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dev190"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2015.04.117"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.02.013"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/cen.13377"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.2217\/fon\u20102018\u20100278"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpurol.2018.02.016"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez043"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/hropen\/hoaa016"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep371"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/PBC.25078"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/del375"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2016.02.2967"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2020.05.009"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.28507"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1530\/EC\u201021\u20100154"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deaa281"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dex306"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deq387"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2017.10.010"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.45.239.13"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2022.01.014"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0164922"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.21780"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0181377"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.23750\/abm.v92i4.11917"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11515"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2009\u20100009"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1586\/17474086.2014.959922"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40738\u2010020\u201000071\u20106"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1159\/000360235"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102605.2000.00240.x"}],"container-title":["Pediatric Blood &amp; Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pbc.30370","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T21:01:03Z","timestamp":1692478863000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pbc.30370"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,7]]},"references-count":39,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1002\/pbc.30370"],"URL":"https:\/\/doi.org\/10.1002\/pbc.30370","archive":["Portico"],"relation":{},"ISSN":["1545-5009","1545-5017"],"issn-type":[{"value":"1545-5009","type":"print"},{"value":"1545-5017","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,7]]},"assertion":[{"value":"2022-04-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e30370"}}