{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T16:26:53Z","timestamp":1792772813121,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Treatment options for advanced intra\u2010abdominal pediatric rhabdomyosarcoma (RMS) with peritoneal sarcomatosis (PS) include cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC). However, optimal dosages and combination regimens of drugs used for HIPEC are underexplored. We aimed to evaluate the efficacy of HIPEC with cisplatin, doxorubicin, and their combination in vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We established PS\/RMS mouse model by intraperitoneally injecting RH30 cells into NOD\/LtSz\u2010scid IL2R\u03b3\u2010null mice. Two weeks post xenotransplantation, mice underwent a single HIPEC procedure at 42\u00b0C for 60\u00a0minutes. Treatment groups received cisplatin (50, 100, and 150\u00a0mg\/m\n                      <jats:sup>2<\/jats:sup>\n                      ) and doxorubicin (30, 45, and 60\u00a0mg\/m\n                      <jats:sup>2<\/jats:sup>\n                      ), administered alone or combined. The control group underwent an intraperitoneal lavage with isotonic saline. Peritoneal cancer index (PCI) was used to quantify the extent of peritoneal tumor spread. Tissue samples were evaluated regarding proliferation (Ki\u201067) and apoptosis (caspase 3).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Mice treated with cisplatin at 100\u00a0mg\/m\n                      <jats:sup>2<\/jats:sup>\n                      (PCI of 3.875,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.007) and 150\u00a0mg\/m\n                      <jats:sup>2<\/jats:sup>\n                      (PCI of 4.556,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.026), and doxorubicin at 30\u00a0mg\/m\n                      <jats:sup>2<\/jats:sup>\n                      (PCI of 2.875,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.001) and 45\u00a0mg\/m\n                      <jats:sup>2<\/jats:sup>\n                      (PCI of 4.143,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.021) showed reduced PCI, with the combination of cisplatin 50\u00a0mg\/m\n                      <jats:sup>2<\/jats:sup>\n                      and doxorubicin 30\u00a0mg\/m\n                      <jats:sup>2<\/jats:sup>\n                      showing the most prominent effect (PCI of 3.333,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.001) compared to the control group (PCI of 8.615). Histologically, there was no difference in Ki\u201067 or caspase 3 expression among the groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The cisplatin\u2010 and doxorubicin\u2010based HIPEC significantly reduces peritoneal tumor dissemination in vivo. Further investigations are needed to explore the underlying molecular responses to optimize therapeutic strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pbc.31366","type":"journal-article","created":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:45:30Z","timestamp":1728348330000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A comparative in vivo study of hyperthermic intraperitoneal chemotherapy with cisplatin versus doxorubicin versus cisplatin plus doxorubicin for the treatment of intra\u2010abdominally disseminated alveolar rhabdomyosarcoma in mice"],"prefix":"10.1002","volume":"71","author":[{"given":"Illya","family":"Martynov","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery and Urology University Hospital Giessen\u2010Marburg  Marburg Germany"},{"name":"Department of Pediatric Surgery University Hospital Giessen\u2010Marburg  Giessen 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