{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T01:13:13Z","timestamp":1773018793498,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study compares and evaluates the clinical utility of three published copy\u2010number alteration (CNA)\u2010 and cytogenetics\u2010integrated risk group classifiers in a prospective cohort of pediatric B\u2010cell acute lymphoblastic leukemia (B\u2010ALL) patients. All cases underwent CNA testing via digital (<jats:italic>n<\/jats:italic> = 185) or conventional multiplex ligation probe amplification (MLPA; <jats:italic>n<\/jats:italic> = 22), and the data were integrated with genetics according to the three classifier systems. Out of 207 pediatric B\u2010ALL patients enrolled, a primary genetic abnormality was noted in 87% (181\/207) of the patients, with high hyperdiploidy being the most common (31%). The overall CNA frequency was 54% (112\/207), with <jats:italic>CDKN2A\/2B<\/jats:italic> deletion being most common (28.5%). Poor\u2010risk CNAs in all three integrated risk classifiers correlated significantly with high TLC (&gt;50\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/L), NCI\u2010HR, MRD&gt;0.01%, ICiCLe\u2010HR, and event. In addition, the two\u2010year EFS and OS were worse for the UKALL\u2010integrated poor\u2010risk group (56.7%; <jats:italic>p =<\/jats:italic> &lt;0.0001\u00a0and 68.3%; <jats:italic>p =<\/jats:italic> 0.012), whereas the digital MLPA\u2010combined poor\u2010risk group and the PersonALL <jats:italic>IKZF1<\/jats:italic> high\u2010risk group had poor two\u2010year EFS (55.3%; <jats:italic>p =<\/jats:italic> 0.038) and (39.5%; <jats:italic>p =<\/jats:italic> 0.015), respectively. Multivariate analysis revealed the UKALL poor\u2010risk integrated group (GEN\u2010PR) to predict relapse (HR: 48.58; <jats:italic>p<\/jats:italic> = 0.014), thereby highlighting that the UKALL\u2010CNA\u2013integrated classifier is more likely to precisely identify a subset of intermediate\u2010risk cytogenetic cases with intermediate\u2010 or poor\u2010risk CNAs that require escalated treatment.<\/jats:p>","DOI":"10.1002\/pbc.31812","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T23:28:24Z","timestamp":1747870104000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Comparative Analysis of Clinical Utility of Cytogenetics and Copy\u2010Number\u2013Integrated Risk Stratification Scores in a Prospective Cohort of Pediatric B\u2010Cell Acute Lymphoblastic Leukemia"],"prefix":"10.1002","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7637-3554","authenticated-orcid":false,"given":"Ajmeera A.","family":"Azeez","sequence":"first","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prateek","family":"Bhatia","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangeetha","family":"Kirubanandhan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rozy","family":"Thakur","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minu","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandeep","family":"Rose","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meenakshi","family":"Malhotra","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharun","family":"Garg","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sreejesh","family":"Sreedharanunni","sequence":"additional","affiliation":[{"name":"Department of Haematology Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manupdesh S.","family":"Sachdeva","sequence":"additional","affiliation":[{"name":"Department of Haematology Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelly","family":"Singla","sequence":"additional","affiliation":[{"name":"Department of Haematology Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parminder","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6970-8272","authenticated-orcid":false,"given":"Richa","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1009-7649","authenticated-orcid":false,"given":"Deepak","family":"Bansal","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9071-7651","authenticated-orcid":false,"given":"Amita","family":"Trehan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Hematology\u2010Oncology Unit Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1400972"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.37.8018"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148972"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-41078-4"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-03-562918"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2017.74.3617"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20818"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018025718"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-019-0423-5"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-023-02309-8"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13063-022-06033-1"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/HS9.0000000000000782"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2021.106683"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13133289"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics13091589"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2017.05.004"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.26281"},{"issue":"4","key":"e_1_2_11_19_1","first-page":"151","article-title":"Gene Copy Number Alterations in Indian Children With B\u2010acute Lymphoblastic Leukemia: Correlation With Survival Outcome","volume":"6","author":"Agarwal M.","year":"2021","journal-title":"PediatrHematolOncol J"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2016.1193855"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2018.01.012"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajco.12400"}],"container-title":["Pediatric Blood &amp; Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pbc.31812","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T12:45:58Z","timestamp":1754916358000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pbc.31812"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,21]]},"references-count":21,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1002\/pbc.31812"],"URL":"https:\/\/doi.org\/10.1002\/pbc.31812","archive":["Portico"],"relation":{},"ISSN":["1545-5009","1545-5017"],"issn-type":[{"value":"1545-5009","type":"print"},{"value":"1545-5017","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,21]]},"assertion":[{"value":"2025-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e31812"}}