{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T10:08:35Z","timestamp":1782382115277,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005677","name":"Jos\u00e9 Carreras Leuk\u00e4mie-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007311","name":"Deutsche Kinderkrebsstiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007311","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013859","name":"Damp Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013859","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Early detection of treatment failure in pediatric patients with mature B\u2010cell non\u2010Hodgkin lymphoma (NHL) could improve treatment stratification. Analysis of circulating tumor DNA (ctDNA) has been established as a biomarker in adult patients with mature B\u2010cell NHL (B\u2010NHL); however, data on ctDNA in pediatric mature B\u2010NHL are lacking.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated genotyping and disease monitoring in initial plasma cell\u2010free DNA (cfDNA) by targeted, normal\u2010matched sequencing in 19 pediatric patients with mature B\u2010NHL, 16 of whom had Burkitt lymphoma or leukemia. Matching lymphoma DNA was available in 18 patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Concentrations of cfDNA were 2.6\u201336\u00a0\u00d7\u00a010<jats:sup>3<\/jats:sup> haploid genome equivalents\/mL plasma (median, 7.8\u00a0\u00d7\u00a010<jats:sup>3<\/jats:sup>). In 10 patients with high\u2010risk disease (risk groups R3 and R4), 60% of somatic short variants were detected in both plasma and lymphoma samples using a tumor\u2010agnostic approach, whereas 26% and 14% were identified in DNA from lymphoma or plasma samples only. In 4\/10 patients with high\u2010risk disease, eight non\u2010silent variants were identified only in the plasma samples. In patients with low\u2010risk disease, 27% of mutations were shared in plasma and tumor; 65% and 8% were detected in DNA from lymphoma or plasma samples only. In a tumor\u2010informed approach, ctDNA was detectable in 16\/18 plasma samples with a proportion of 0.045%\u201355%, demonstrating its potential for minimal disease monitoring.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Non\u2010invasive genotyping from a plasma sample is feasible in high\u2010risk pediatric mature B\u2010cell lymphoma patients. Our findings provide the basis for investigating the clinical utility of non\u2010invasive lymphoma genotyping and disease monitoring in pediatric mature B\u2010NHL.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.31895","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T07:54:41Z","timestamp":1751615681000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Circulating Tumor DNA in Pediatric Mature B\u2010Cell Non\u2010Hodgkin Lymphoma for Genotyping and Minimal Disease Monitoring"],"prefix":"10.1002","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6399-6960","authenticated-orcid":false,"given":"Jana","family":"Werner","sequence":"first","affiliation":[{"name":"Pediatric Hematology and Oncology and NHL\u2010BFM Study Centre University Medical Centre Hamburg\u2010Eppendorf  Hamburg 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