{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T22:40:17Z","timestamp":1778539217962,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018675","name":"Kids' Cancer Project","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018675","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Pulmonary complications due to infection contribute significantly to post\u2010haematopoietic stem cell transplant (HCT) morbidity and mortality. Standard microbiological investigations, when performed on bronchoalveolar lavage (BAL) fluid, can take days to weeks to confirm a diagnosis. We aimed to determine the diagnostic performance of the BioFire FilmArray pneumonia panel <jats:italic>plus<\/jats:italic> (FA\u2010PP), a multiplex polymerase chain reaction (PCR) panel that detects 18 bacterial and nine viral targets, when applied to BAL obtained by flexible bronchoscopy, in children undergoing HCT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed a single\u2010centre prospective observational study in children undergoing allogeneic HCT, who underwent BAL pre\u2010 and post\u2010HCT. To determine the diagnostic performance of the FA\u2010PP, we measured the positive and negative concordance, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV), compared with standard microbiological investigations, which was considered the gold standard. The clinical impact of the FA\u2010PP was also qualitatively measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This study enrolled 16 children who had 31 BAL samples collected, both pre\u2010 and post\u2010HCT. In total, there were seven patients who underwent eight BALs while symptomatic, and 50% (4\/8) of these results were concordant between FA\u2010PP and standard microbiological investigations. In the 13 patients who had 23 BALs collected while asymptomatic, 78% (18\/23) of these results were concordant. In the cohort as a whole, sensitivity, specificity, PPV and NPV were 100%, 78%, 54% and 100%, respectively. In 50% (4\/8) of patients who underwent a BAL while symptomatic, the FA\u2010PP resulted in a hypothetical or actual clinical change, compared to 22% (5\/23) of patients who underwent an asymptomatic BAL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In conclusion, we report the first prospective evaluation of the diagnostic performance of the FA\u2010PP in BAL, in a high\u2010risk paediatric HCT cohort. We demonstrate that the FA\u2010PP is a potentially useful adjunct to traditional standard microbiological investigations that can provide clinically impactful diagnostic information.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pbc.31940","type":"journal-article","created":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T05:34:34Z","timestamp":1754112874000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Utility of BioFire FilmArray Pneumonia Panel <i>Plus<\/i> in a High\u2010Risk Cohort: Children Post Allogeneic Stem Cell Transplantation"],"prefix":"10.1002","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3851-7502","authenticated-orcid":false,"given":"Hannah","family":"Walker","sequence":"first","affiliation":[{"name":"Children's Cancer Centre Royal Children's Hospital  Parkville Australia"},{"name":"Department of Paediatrics University of Melbourne  Parkville Australia"},{"name":"Murdoch Children's Research Institute  Parkville Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theresa","family":"Cole","sequence":"additional","affiliation":[{"name":"Children's Cancer Centre Royal Children's Hospital  Parkville Australia"},{"name":"Department of Paediatrics University of Melbourne  Parkville Australia"},{"name":"Murdoch Children's Research Institute  Parkville Australia"},{"name":"Allergy and Immunology Royal Children's Hospital  Parkville Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liam","family":"Gubbels","sequence":"additional","affiliation":[{"name":"Murdoch Children's Research Institute  Parkville Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Pham","sequence":"additional","affiliation":[{"name":"Laboratory Services Royal Children's Hospital  Parkville Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Neeland","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University of Melbourne  Parkville Australia"},{"name":"Murdoch Children's Research Institute  Parkville Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane","family":"Hanna","sequence":"additional","affiliation":[{"name":"Children's Cancer Centre Royal Children's Hospital  Parkville Australia"},{"name":"Department of Paediatrics University of Melbourne  Parkville Australia"},{"name":"Murdoch Children's Research Institute  Parkville Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Clifford","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University of Melbourne  Parkville Australia"},{"name":"Murdoch Children's Research Institute  Parkville Australia"},{"name":"Laboratory Services Royal Children's Hospital  Parkville Australia"},{"name":"Department of Infection Diseases Royal Children's Hospital  Parkville Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6000-3180","authenticated-orcid":false,"given":"Shivanthan","family":"Shanthikumar","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University of Melbourne  Parkville Australia"},{"name":"Murdoch Children's Research Institute  Parkville Australia"},{"name":"Respiratory and Sleep Medicine Royal Children's Hospital  Parkville Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabrielle M.","family":"Haeusler","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University of Melbourne  Parkville Australia"},{"name":"Murdoch Children's Research Institute  Parkville Australia"},{"name":"Department of Infection Diseases Royal Children's Hospital  Parkville Australia"},{"name":"Department of Infectious Diseases Peter MacCallum Cancer Centre  Melbourne Australia"},{"name":"NHMRC National Centre for Infections in Cancer Sir Peter MacCallum Department of Oncology University of Melbourne  Parkville Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cti2.70002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cti2.70003"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.755878"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000003648"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000883"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2014.09.019"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2015.06.002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.03.055"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-12-617035"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00343-20"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00135-20"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jpids\/piae014"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000004349"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874279301810010015"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000004629"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2022.09.003"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-024-02999-4"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020009246"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16452"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtct.2021.09.010"}],"container-title":["Pediatric Blood &amp; Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pbc.31940","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T03:39:30Z","timestamp":1756179570000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pbc.31940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":20,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1002\/pbc.31940"],"URL":"https:\/\/doi.org\/10.1002\/pbc.31940","archive":["Portico"],"relation":{},"ISSN":["1545-5009","1545-5017"],"issn-type":[{"value":"1545-5009","type":"print"},{"value":"1545-5017","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"2025-05-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e31940"}}