{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T19:05:25Z","timestamp":1786820725351,"version":"build-2736575974"},"reference-count":19,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Kaposiform lymphangiomatosis (KLA) is a complex lymphatic anomaly characterized by lymphatic overgrowth and dysfunction. The goal of this study was to determine how the <jats:italic>NRAS<jats:sup>Q61R<\/jats:sup><\/jats:italic> variant found in the tissue of KLA patients impacts key characteristics of lymphatic endothelial cells (LEC). LEC expressing NRAS<jats:sup>Q61R<\/jats:sup> had a spindled morphology, increased migration, increased proliferation, increased MAPK signaling, and elevated angiopoietin\u20102 production. In an in vitro lymphangiogenesis model, LEC expressing NRAS<jats:sup>Q61R<\/jats:sup> showed increased sprouting and formed enlarged and disorganized vascular structures. This model of LEC expressing NRAS<jats:sup>Q61R<\/jats:sup> can be used to give insights into KLA pathogenesis and test new treatments.<\/jats:p>","DOI":"10.1002\/pbc.31984","type":"journal-article","created":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T06:35:38Z","timestamp":1755585338000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Human Lymphatic Endothelial Cells Expressing NRAS<sup>Q61R<\/sup> Model Characteristics of Kaposiform Lymphangiomatosis"],"prefix":"10.1002","volume":"72","author":[{"given":"Dermot A.","family":"Fox","sequence":"first","affiliation":[{"name":"Division of Pulmonary Biology Cincinnati Children's Hospital Medical Center and Department of Pediatrics University of Cincinnati College of Medicine  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Griffin","family":"McDaniel","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Biology Cincinnati Children's Hospital Medical Center and Department of Pediatrics University of Cincinnati College of Medicine  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Pastura","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Biology Cincinnati Children's Hospital Medical Center and Department of Pediatrics University of Cincinnati College of Medicine  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8538-2703","authenticated-orcid":false,"given":"Timothy D.","family":"Le Cras","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Biology Cincinnati Children's Hospital Medical Center and Department of Pediatrics University of Cincinnati College of Medicine  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.31605"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41436-018-0390-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.30219"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2013.10.013"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-022-09836-7"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.31032"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.6.797"},{"key":"e_1_2_8_9_1","article-title":"Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis","volume":"3","author":"Nakatsu M. 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