{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T01:21:47Z","timestamp":1769131307276,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,5,4]],"date-time":"2007-05-04T00:00:00Z","timestamp":1178236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Three spectrophotometric methods for the quantitative determination of different flavonoid groups and total phenolics in Croatian propolis samples were optimised and validated. The assay based on the formation of aluminium chloride complex (with galangin as a standard) was applied to the quantification of flavones and flavonols, while the 2,4\u2010dinitrophenylhydrazine method (with pinocembrine as a reference) was used for the quantification of flavanones. Total phenolic content was measured by the Folin\u2013Ciocalteau method using reference solution of caffeic acid:galangin:pinocembrine (1:1:1). Through analytical validation, the most suitable extraction conditions (with respect to time, temperature and concentration of extraction solvent) were determined, and final conditions for the extraction were established (80% ethanol, 1 h at the room temperature). The appropriate ratio between the mass of raw propolis and the extraction solvent volume was also established. By the application of the optimised method of extraction, 10 propolis tinctures were prepared and subjected to the analysis of general pharmacopoeial parameters, which are fundamental for the creation of quality specification (relative density, dry residue of extract, content of ethanol, methanol and 2\u2010propanol). Additionally, the content of waxes as the main inactive constituents was determined in order to observe the level of their migration from crude propolis to the prepared tinctures. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.1001","type":"journal-article","created":{"date-parts":[[2007,5,4]],"date-time":"2007-05-04T08:42:48Z","timestamp":1178268168000},"page":"451-459","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["Optimisation of an extraction procedure and chemical characterisation of Croatian propolis tinctures"],"prefix":"10.1002","volume":"18","author":[{"given":"J.","family":"Cvek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Medi\u0107\u2010\u0160ari\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Jasprica","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Zub\u010di\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Vitali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Mornar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Vedrina\u2010Dragojevi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Tomi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2005.05.004"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/0005772X.2000.11099492"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:2000102"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1029"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/009286159803200114"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1515\/znc-1994-11-1204","article-title":"Phenolic composition of propolis from China and from South America","volume":"49","author":"Bonvehi JS","year":"1994","journal-title":"Z Naturforsch"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(97)00145-2"},{"key":"e_1_2_1_9_1","first-page":"178","article-title":"Estimation of total flavonoid content in propolis by two complementary colorimetric methods","volume":"10","author":"Chang CC","year":"2002","journal-title":"J Food Drug Anal"},{"key":"e_1_2_1_10_1","unstructured":"1996 CHEMID A chemical database sponsored by the National Library of Medicine"},{"key":"e_1_2_1_11_1","first-page":"570","volume-title":"European Pharmacopoeia","author":"Directorate for the Quality of Medicines of the Council of Europe","year":"2004"},{"key":"e_1_2_1_12_1","first-page":"222","volume-title":"European Pharmacopoeia","author":"Directorate for the Quality of Medicines of the Council of Europe","year":"2004"},{"key":"e_1_2_1_13_1","first-page":"239","volume-title":"European Pharmacopoeia","author":"Directorate for the Quality of Medicines of the Council of Europe","year":"2004"},{"key":"e_1_2_1_14_1","unstructured":"European Agency for the Evaluation of Medicinal Products.2005.Guideline on Specifications: Test Procedures and Acceptance Criteria for Herbal Substances Herbal Preparations and Herbal Medicinal Products\/Traditional Herbal Medicinal Products (EMEA\/CVMP\/815\/00 Rev 1). 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Analysis of propolis: some parameters and procedures for chemical quality control","volume":"37","author":"Woiski RG","year":"1998","journal-title":"J Apic Res"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpca.1001","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.1001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T12:56:08Z","timestamp":1761051368000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.1001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,4]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1002\/pca.1001"],"URL":"https:\/\/doi.org\/10.1002\/pca.1001","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"value":"0958-0344","type":"print"},{"value":"1099-1565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,4]]},"assertion":[{"value":"2006-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-03-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-05-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}