{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:42:12Z","timestamp":1789710132989,"version":"4.0.1"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,2,20]],"date-time":"2008-02-20T00:00:00Z","timestamp":1203465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Extracts of <jats:italic>Tripterygium wilfordii<\/jats:italic> Hook F. have been widely used in China to treat a variety of autoimmune and inflammatory diseases. The diterpenoids triptolide and tripdiolide are two major active components in the <jats:italic>T. wilfordii<\/jats:italic> ethyl acetate extract. An efficient solid\u2010phase extraction and high\u2010performance liquid chromatography (SPE\u2010HPLC) method to measure triptolide content in the extract has been previously reported. However, a suitable means of tripdiolide quantification is not available because of interfering compounds in the extract that co\u2010elute with tripdiolide. Therefore, this paper describes a method wherein tripdiolide content can be measured from a small amount of the extract. The extract solution (600 \u00b5L) was applied into an aminopropyl SPE tube. Triptolide was eluted with dichloromethane:methanol (1 mL, 49:1 v\/v), followed by tripdiolide elution with dichloromethane:methanol (3 mL, 17:3 v\/v). The tripdiolide eluate was analysed by HPLC using an isocratic solvent system and was quantified by measuring the peak area at 219 nm. The contents of triptolide and tripdiolide in the extract were determined to be 807.32 \u00b1 51.94 and 366.13 \u00b1 17.21 \u00b5g\/g of extract, respectively. Since tripdiolide is biologically active and makes up a considerable portion of the extract, for extract quality control and standardisation purposes, it should be measured along with triptolide using the proposed SPE\u2010HPLC method. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.1059","type":"journal-article","created":{"date-parts":[[2008,2,20]],"date-time":"2008-02-20T12:25:14Z","timestamp":1203510314000},"page":"348-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Determination of tripdiolide in root extracts of <i>Tripterygium wilfordii<\/i> by solid\u2010phase extraction and reversed\u2010phase high\u2010performance liquid chromatography"],"prefix":"10.1002","volume":"19","author":[{"given":"Jun","family":"Ma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara M.","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Poulev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilya","family":"Raskin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,6,16]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.02.083"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826079408013632"},{"key":"e_1_2_1_4_1","volume-title":"American College of Rheumatology Proceedings of 2006 Annual Meeting","author":"Goldbach\u2010Mansky RT","year":"2006"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/recl.19961150115"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)80913-9"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-0172(97)80040-6"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2007.02.021"},{"key":"e_1_2_1_9_1","first-page":"539","article-title":"Comparison of active constituent contents in common threewingnut (Tripterygium Hook F) from different regions","volume":"25","author":"Nie K","year":"1994","journal-title":"Chin Trad Herb Drugs"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-006-0156-8"},{"key":"e_1_2_1_11_1","first-page":"1567","article-title":"Determination of tripdiolide in Tripterygium wilfordii by capillary gas chromatography","volume":"36","author":"Rao Y","year":"2005","journal-title":"Chin Trad Herb Drugs"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.692"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780341011"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/S0022-3565(25)24561-X","article-title":"The identity of immunosuppressive components of the ethyl acetate extract and chloroform methanol extract (T2) of Tripterygium wilfordii Hook F","volume":"272","author":"Tao X","year":"1995","journal-title":"J Pharmac Exp Ther"},{"key":"e_1_2_1_15_1","first-page":"2160","article-title":"A phase I study of ethyl acetate extract of the Chinese antirheumatic herb Tripterygium wilfordii Hook F in rheumatoid arthritis","volume":"28","author":"Tao X","year":"2001","journal-title":"J Rheumatol"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10411"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpca.1059","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.1059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T11:54:51Z","timestamp":1761047691000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.1059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6,16]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,7]]}},"alternative-id":["10.1002\/pca.1059"],"URL":"https:\/\/doi.org\/10.1002\/pca.1059","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"value":"0958-0344","type":"print"},{"value":"1099-1565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,6,16]]},"assertion":[{"value":"2007-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-12-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}