{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T16:10:06Z","timestamp":1761063006887,"version":"build-2065373602"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,5,22]],"date-time":"2008-05-22T00:00:00Z","timestamp":1211414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Introduction: <\/jats:bold>Over the last decade HPLC\u2010NMR has become a robust analytical technique that has been applied to a wide range of studies, particularly plant extracts. There have been only a few applications of the use of HPLC\u2010NMR to profile marine natural product extracts and no studies involving marine algae. The marine alga selected for this study belongs to the genus <jats:italic>Plocamium<\/jats:italic>, which is a well known source of polyhalogenated monoterpenes.<\/jats:p><jats:p><jats:bold>Objective: <\/jats:bold>To chemically profile the marine alga <jats:italic>P<\/jats:italic>.<jats:italic> mertensii<\/jats:italic>, using a combination of on\u2010line (HPLC\u2010NMR) and off\u2010line approaches.<\/jats:p><jats:p><jats:bold>Methodology: <\/jats:bold><jats:italic>P<\/jats:italic>.<jats:italic> mertensii<\/jats:italic> was extracted with 3:1 methanol\u2013dichloromethane and subsequently partitioned into dichloromethane and methanol\u2010soluble fractions. The methanol partition was exclusively profiled by HPLC\u2010NMR (on\u2010flow, stop\u2010flow and time\u2010slice experiments) whilst the dichloromethane partition was investigated by conventional isolation and purification procedures.<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold>HPLC\u2010NMR analysis of the methanol partition partially identified the presence of the major compounds <jats:bold>7<\/jats:bold>, <jats:bold>13<\/jats:bold>, <jats:bold>27<\/jats:bold> and <jats:bold>37<\/jats:bold>, the structures of which were unequivocally elucidated by off\u2010line characterisation of the dichloromethane partition. Two additional minor metabolites (<jats:bold>3<\/jats:bold> and <jats:bold>8<\/jats:bold>) present in the dichloromethane partition were only tentatively identified as these co\u2010occurred in a mixture with compounds <jats:bold>7<\/jats:bold> and <jats:bold>13<\/jats:bold>. As a result of this study a number of chemical shift reassignments were made for compound <jats:bold>37<\/jats:bold>.<\/jats:p><jats:p><jats:bold>Conclusion: <\/jats:bold>This is one of the few reports describing the application of HPLC\u2010NMR to rapidly profile or dereplicate a marine organism and the first application of HPLC\u2010NMR to successfully profile the chemistry of a marine alga. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.1075","type":"journal-article","created":{"date-parts":[[2008,5,22]],"date-time":"2008-05-22T09:16:48Z","timestamp":1211447808000},"page":"453-470","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Phytochemical analysis of the Southern Australian marine alga, <i>Plocamium mertensii<\/i> using HPLC\u2010NMR"],"prefix":"10.1002","volume":"19","author":[{"given":"Daniel","family":"Dias","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia","family":"Urban","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,5,22]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/np50064a026"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1999.4093"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/np960537j"},{"key":"e_1_2_1_5_1","first-page":"610","article-title":"A new polyhalogenated epoxymonoterpene from Plocamium cartilagineum","volume":"37","author":"Abreu PM","year":"1998","journal-title":"Indian J Chem Sect B"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/0470854820"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf025857p"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006901803636"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(01)95240-2"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00289-9"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1071\/CH9840537"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/np50034a030"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/np050504g"},{"key":"e_1_2_1_14_1","first-page":"1743","article-title":"Tropical marine algae. 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