{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T22:32:54Z","timestamp":1787956374193,"version":"build-2784847793"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,3,3]],"date-time":"2009-03-03T00:00:00Z","timestamp":1236038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2009,5]]},"abstract":"Abstract<\/jats:title>\n \n Introduction<\/jats:title>\n Platycosides, the primary constituents of Platycodi Radix, are known to have numerous and varied biological activities, exerting anti\u2010inflammation, anti\u2010allergy, anti\u2010tumour, anti\u2010obesity and anti\u2010hyperlipidemia effects. However, effective methods for isolating and purifying platycosides from Platycodi Radix are not currently available.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n To develop an efficient method for the preparative separation of six platycosides from Platycodi Radix by high\u2010speed counter\u2010current chromatography (HSCCC) coupled with an evaporative light scattering detection (ELSD) system.<\/jats:p>\n <\/jats:sec>\n \n Methodology<\/jats:title>\n \n Preparative separation was performed by water extraction using reversed\u2010phase C\n 18<\/jats:sub>\n column chromatography on an HSCCC\u2010ELSD system. A two\u2010phase solvent system comprised hexane\u2013\n n<\/jats:italic>\n \u2010butanol\u2013water (1:40:20, v\/v) and (1:10:5, v\/v) was employed. Two other key parameters, revolution speed of the separation column and flow\u2010rate of the mobile phase, were also investigated for optimum HSCCC performance. Each peak fraction obtained from separation of the platycosides was collected according to the ELSD elution profile and determined by HPLC.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Using the described method, six platycosides, all with purities of over 94%, could be isolated from 300\u00a0mg of the platycoside\u2010enriched fraction. Their structures were characterized by electrospray ionisation mass spectrometry (ESI\u2010MS),\n 1<\/jats:sup>\n H\u2010NMR and\n 13<\/jats:sup>\n C\u2010NMR.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Six of the main bioactive platycosides in Platycodi Radix could be isolated and purified systematically by HSCCC. Copyright \u00a9 2009 John Wiley & Sons, Ltd.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/pca.1116","type":"journal-article","created":{"date-parts":[[2009,3,3]],"date-time":"2009-03-03T04:57:06Z","timestamp":1236056226000},"page":"207-213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Preparative isolation of six major saponins from Platycodi Radix by high\u2010speed counter\u2010current chromatography"],"prefix":"10.1002","volume":"20","author":[{"given":"Young Wan","family":"Ha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeong Shik","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,3,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.10.042"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X04001916"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.08.008"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00268a001"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.54.557"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.54.1285"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.09.038"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.09.015"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.01.038"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.8.2241"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","unstructured":"IshiiH ToriK TozyoTandYoshimuraY.1984. 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