{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:52:04Z","timestamp":1761094324327,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T00:00:00Z","timestamp":1269302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Introduction \u2013 A large number of natural and synthetic compounds having butenolides as a core unit have been described and many of them display a wide range of biological activities. Butenolides from <jats:italic>P. malacophyllum<\/jats:italic> have presented potential antifungal activities but no specific, fast, and precise method has been developed for their determination.<\/jats:p><jats:p>Objective \u2013 To develop a methodology based on micellar electrokinetic chromatography to determine butenolides in <jats:italic>Piper<\/jats:italic> species.<\/jats:p><jats:p>Methodology \u2013 The extracts were analysed in an uncoated fused\u2010silica capillaries and for the micellar system 20\u2009mmol\/L SDS, 20% (v\/v) acetonitrile (ACN) and 10\u2009mmol\/L STB aqueous buffer at pH 9.2 were used. The method was validated for precision, linearity, limit of detection (LOD) and limit of quantitation (LOQ) and the standard deviations were determined from the standard errors estimated by the regression line.<\/jats:p><jats:p>Results \u2013 A micellar electrokinetic chromatography (MEKC) method for determination of butenolides in extracts gave full resolution for <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold>. The analytical curve in the range 10.0\u201350.0\u2009\u00b5g\/mL (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> = 0.999) provided LOD and LOQ for <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold> of 2.1\/6.3 and 1.1\/3.5\u2009\u00b5g\/mL, respectively. The RSD for migration times were 0.12 and 1.0% for peak area ratios with 100.0 \u00b1 1.4% of recovery.<\/jats:p><jats:p>Conclusions \u2013 A novel high\u2010performance MEKC method developed for the analysis of butenolides <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold> in leaf extracts of <jats:italic>P. malacophyllum<\/jats:italic> allowed their quantitative determined within an analysis time shorter than 5\u2009min and the results indicated CE to be a feasible analytical technique for the quantitative determination of butenolides in <jats:italic>Piper<\/jats:italic> extracts. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.1213","type":"journal-article","created":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T02:28:33Z","timestamp":1269311313000},"page":"428-433","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development and Validation of a Micellar Electrokinetic Chromatography Method for Quantitative Determination of Butenolides in <i>Piper malacophyllum<\/i> (C. Presl) C. 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