{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T17:10:11Z","timestamp":1761066611421,"version":"build-2065373602"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T00:00:00Z","timestamp":1269302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acetonitrile\u2010based HPLC systems are the most commonly used for carotenoid analysis from different plant tissues. Because of the acetonitrile shortage, an HPLC system for the separation of carotenoids on C<jats:sub>18<\/jats:sub> reversed\u2010phase columns was developed in which an acetonitrile\u2013alcohol\u2010based mobile phase was replaced by nitromethane. This solvent comes closest to acetonitrile with respect to its elutrophic property. Our criterion was to obtain similar separation and retention times for a range of differently structured carotenoids. This was achieved by further increase in the lipophilicity with ethylacetate. For all the carotenoids which we tested, we found co\u2010elution only of <jats:italic>\u03b2<\/jats:italic>\u2010cryptoxanthin and lycopene. By addition of 1% of water, separation of this pair of carotenoids was also achieved. The final recommended mobile phase consisted of nitromethane\u2009:\u20092\u2010propanol\u2009:\u2009ethyl acetate\u2009:\u2009water (79\u2009:\u200910\u2009:\u200910\u2009:\u20091, by volume). On Nucleosil C<jats:sub>18<\/jats:sub> columns and related ones like Hypersil C<jats:sub>18<\/jats:sub>, we obtained separation of carotenes, hydroxyl, epoxy and keto derivatives, which resembles the excellent separation properties of acetonitrile\u2010based mobile phases on C<jats:sub>18<\/jats:sub> reversed phase columns. We successfully applied the newly developed HPLC system to the separation of carotenoids from different vegetables and fruit. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.1214","type":"journal-article","created":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T01:42:38Z","timestamp":1269308558000},"page":"434-437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A nitromethane\u2010based HPLC system alternative to acetonitrile for carotenoid analysis of fruit and vegetables"],"prefix":"10.1002","volume":"21","author":[{"given":"Gerhard","family":"Sandmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,8,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb15000.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ern210"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.09.077"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.049817"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1201\/9780203910214"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf020539b"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(93)83467-7"},{"key":"e_1_2_6_9_1","first-page":"145","volume-title":"Carotenoids Volume 1A, Chromatography: Part IV","author":"Pfander H","year":"1995"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.2533\/chimia.1980.179","article-title":"Trennung von Carotinen und Diterpenen mit Hochleistungsfl\u00fcssigchromatographie","volume":"34","author":"Pfander H","year":"1980","journal-title":"Chimia"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf902113n"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:BILE.0000025880.91269.cf"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-003-0545-4"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00411017"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00502-0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809737105"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpca.1214","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpca.1214","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.1214","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T16:29:01Z","timestamp":1761064141000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.1214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8,25]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1002\/pca.1214"],"URL":"https:\/\/doi.org\/10.1002\/pca.1214","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"type":"print","value":"0958-0344"},{"type":"electronic","value":"1099-1565"}],"subject":[],"published":{"date-parts":[[2010,8,25]]},"assertion":[{"value":"2009-11-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-01-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-08-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}