{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:42:37Z","timestamp":1773322957023,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,8,26]],"date-time":"2010-08-26T00:00:00Z","timestamp":1282780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Introduction \u2013 Mailuoning is prepared from a traditional formula of Chinese medicines and widely used as an antithrombotic agent. In this study, the platelet binding assay was used as a novel biospecific separation and analysis method to explore its active constituents, which could be considered as marker compounds for quality control.<\/jats:p><jats:p>Objective \u2013 To establish a rapid and simple method to predict marker compounds in herbal medicine injection and evaluate the effects of those compounds.<\/jats:p><jats:p>Material and methods \u2013 Platelets were used to bind and separate constituents. Binding constituents were analysed and taken as potential active compounds for further evaluation. Solid\u2010phase\u2010extraction was adopted to improve sensitivity. HPLC\u2010DAD and ESI\u2010MS were used to determine the binding constituents.<\/jats:p><jats:p>Results \u2013 Five compounds were extracted through the platelet binding process and identified as neochlorogenic acid, caffeic acid, isochlorogenic acid and their isomers. Caffeic acid was selected for the flow cytometric assay to test its effect on platelets activation, which was determined by CD62P (P\u2010selectin) expression. The results indicated that caffeic acid could significantly inhibit platelet activation while chlorogenic acid did not.<\/jats:p><jats:p>Conclusion \u2013 Caffeic acid could be considered as a marker compound of Mailuoning injection due to its anti\u2010platelet effect. The study also suggested that platelet binding assay combined with some preconcentration technique could be efficiently used to predict anti\u2010platelet compounds in complicated herbal medicines. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.1260","type":"journal-article","created":{"date-parts":[[2010,8,29]],"date-time":"2010-08-29T21:50:38Z","timestamp":1283118638000},"page":"87-93","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Analysis of marker compounds with anti\u2010platelet aggregation effects in mailuoning injection using platelet binding assay combined with HPLC\u2010DAD\u2010ESI\u2010MS and solid\u2010phase extraction 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